Determination of Myostatin Gene polymorphisms and alleles frequencies in Broiler chicken

	Determination of Myostatin Gene polymorphisms and alleles frequencies in Broiler chicken
iraqi poultry sciences journal
Article 1, Volume 10, Issue 2, 2016, Pages 35-43
Abstract
This study was conducted at the poultry farm of Animal production Department at College of Agriculture University of Qadisiyah during the period from 9/4/2015 to 20/5/20015 and in a Laboratory dealing with the analysis of molecular genetic in College of Agriculture University of Baghdad. The objective of this study was to identify the genotypes for Myostatin gene (GDF-8) of Ross broiler, using three type of Restriction enzymes (Aci I, Bbv I ,Bbs I), by technique the Restriction fragment length polymorphisms (RFLPs). The results of this study can summarized as follows. The percentage of genotype distribution for the Myostatin gene by Restriction enzyme Aci I in sample of studied was 32, 54 and 14 % for the genotypes. GG, GA and AA respectively and the differences between these percentages was highly significant. The alleles frequencies for alleles (G) was 0.59 while the alleles frequencies for alleles (A) was 0.41 according to the analysis of Myostatin gene (GDF-8) in this study. The percentage of genotype distribution for the Myostatin gene by Restriction enzyme Bbs I in sample was 6.78, 86 and 31.36 % for the genotypes. CC, CT and TT respectively and the differences between these percentages was highly significant. The alleles frequencies for alleles (C) was 0.38 and (T) was 0.62 respectively according to the analysis of Myostatin gene (GDF- 8) in this study. The percentage of genotype distribution for the Myostatin gene by Restriction enzyme Bbv I in sample of studied was 98 and 2 % for the genotypes. AA and GA respectively. The allele’s frequencies for alleles (A) was 0.98 and (G) was 0.02 respectively according to the analysis of Myostatin gene (GDF-8) in this study.

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