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The detection of human papilloma virus- 16, in relation to P53 in oral squamous cell carcinoma by in situ hybridization | ||
| Journal of baghdad college of dentistry | ||
| Article 1, Volume 21, Issue 4, 2009, Pages 80-85 | ||
| Authors | ||
| Ban A. Abdul. Majeed; Kadhim Al-Soudani; Maha M. Al-Sened | ||
| Abstract | ||
| ABSTRACTBackground: Squamous cell carcinoma of the oral cavity constituted a major health problem and can be a leadingcause of death. Several studies link high risk Human papillomavirus -16 to oral squamous cell carcinoma as animportant etiological factor. Several molecular markers have also been the subject of an intense research work,trying to clarify their role in oral carcinogenesis. Of these, the cancer suppresser gene P53 has been extensivelystudied. Aim of the study: 1. to detect the association of the molecular markers Human papillomavirus -16, in relationto P53 in the oral Squamous cell carcinoma using in situ hybridization technique. 2. To correlate Humanpapillomavirus -16 with clinical data (age, sex, risk factors grade &stage).Material & Methods: Tumor tissues from 33 patients with newly diagnosed as oral squamous cell carcinoma & whowere surgically treated collected, formalin fixed & paraffin embedded. Sections on charged slides were made fromeach tissue block and submitted to in situ hybridization technique utilizing Human papillomavirus -16, P53 cDNAprobes.Results: Human papillomavirus -16 was localized by in situ hybridization specifically within the nuclei of cancer cell itwas detected in (27) (81.8%) of 33 oral squamous cell carcinoma patients. By using in situ hybridization P53 weredetected in (93.9%) cases. There was no significant correlation between the Human papillomavirus -16 &P53positivity. Most of Human papillomavirus -16 positive patients were within the age group (50-59) years. However, therewas no significant correlation between Human papillomavirus -16 positive &age. No significant correlation wasfound to sex &risk factors either. Most of cases were moderately differentiated squamous cell carcinoma (79%).however no significant correlation was detected regarding histopathological grading or clinical staging with Humanpapillomavirus -16, & P53.Conclusions: Being highly associated with Human papillomavirus -16 must be searched for in cancer tissues of suchpatients.Keywords: Oral squamous cell carcinoma, Human papillomavirus -16, P53, in situ hybridization. (J Bagh Coll Dentistry2009; 21(4): 80-85). | ||
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