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13- STUDY THE EFFECT OF DIFFERENT CULTURE MEDIA ON SOME FROZEN SEMEN CHARACTERISTICS OF IRAQI BLACK LOCAL GOAT SPERMATOZOA | ||
| Basrah Journal of Veterinary Research | ||
| Article 13, Volume 17, Issue 1, 2018, Pages 147-154 PDF (0 K) | ||
| DOI: 10.33762/bvetr.2018.144947 | ||
| Authors | ||
| Taher A.Fahad; Khairi G. Al-Rikabi; Abdulrazak N. Khudair | ||
| Abstract | ||
| The semen was collected from five adult Iraqi black local bucks by the artificial vagina and using estrous induced doe the semen was diluted with egg yolk 10% extender and frozen by liquid nitrogen for three months, macroscopic and microscopic evaluation were done after collection, dilution and thawing, three media(TCM-199, DMEM and SOF)were used to see the their effect on activation of spermatozoa. The results showed that TCM-199 media presented higher significance in sperm motility, progressive motility compared with DMEM and SOF media. There was a great decrease in the concentration of spermatozoa after activation using these media which didn’t differ significantly among them and they were higher significantly compared with control in normal morphology. | ||
| Keywords | ||
| Bucks; DMEM; Sperm motility | ||
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