- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Study the Antibacterial Activity of Bacteriocin Produced from the Locally Isolated Cronobacter sakazakii | ||
| Journal of Al-Nahrain University - Science | ||
| Article 1, Volume 21, Issue 2, 2018, Pages 112-118 | ||
| Authors | ||
| Hameed M. Jasim; Nedhaal S. Zbar; Mahaba R. Al-Roubaiee | ||
| Abstract | ||
| Cronobacter sakazakii was isolated from food samples and identified using morphological, biochemical tests and VITEK-2 system to produce bacteriocin by induction with mitomycin-C. C.sakazakii isolates were screened for their ability for bacteriocin production. Result showed that all these isolates were bacteriocin producers, but the most efficient isolate was MA7. Antibacterial activity of bacteriocin against some pathogenic bacteria was tested using well diffusion method. Bacteriocin was partially purified by ammonium sulfate precipitation, ion exchange using (DEAE)-Cellulose. After purification steps, three peaks were obtained. quantitative screening of bacteriocin involving the estimation of protein concentration and estimation of antibacterial activity showed that the large first peak was 1.6 mg/ml, 640 U/ml. | ||
| Keywords | ||
| Cronobacter sakazakii; Bacteriocin; Purification | ||
|
Statistics Article View: 116 PDF Download: 85 |
||