Determination of the antibacterial activity of MTA, and pulpotec filling materials against selected microorganisms (in vitro study)

A. Mahmood, Maha

	Determination of the antibacterial activity of MTA, and pulpotec filling materials against selected microorganisms (in vitro study)
Journal of baghdad college of dentistry
Article 1, Volume 22, Issue 4, 2010, Pages 115-118
Author
Maha A. Mahmood
Abstract
ABSTRACTBackground: This in vitro study concerned with the assessment of the antibacterial effects of two materials used asa pulpotomy medicament, mineral trioxide aggregate (MTA) and a new filling material Pulpotec (PD) on variousspecies of microorganisms, using agar diffusion test.Materials and methods: Abase layer of petri plates was made using Muller-Hinton agar. After solidification, twocavities were made in agar and filled with fresh mixed materials. Staphylococcus aureus, Enterococcus Faecalis,and Escherichia coli were seeded by pour plate. The plates were preincubated for 2 hours at room temperaturefollowed by incubation at 37◦C. The inhibition zone diameters were measured in (mm) at 24, 48 and 72 hours. Datawere analyzed statistically using descriptive and analytic statistics, the descriptive including mean and standarddeviation, and the analytic statistics in which t-test was run for multiple comparisons (between materials, andmicroorganisms).Results: The highest mean diameters of growth inhibition zones were observed around PD against all testedmicroorganisms over 72 hours. According to t-test, there was a high significant difference between PD in comparisonwith MTA (P< 0.01). The difference was highly significance between the tested microorganisms.Conclusion: The in vitro antibacterial activity of pulpotec offers additional advantage over MTA.Key words: antibacterial agent, mineral trioxide aggregate, pulpotec, pulpotomy. (J Bagh Coll Dentistry2010;22(4):115-118).

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