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Assessment of Vitamin D Receptor Gene Expression In Type 2 Diabetic and HypertensivePatients | ||
| Albahir journal | ||
| Article 1, Volume 6, Issue 11, 2017, Pages 107-117 | ||
| Author | ||
| Zuhair Mohammed Ali Jeddoaa | ||
| Abstract | ||
| Vitamin D receptor (encoded by the VDR gene) is a transcription factor from the nuclear receptor subfamily. It has been reported that this gene has been participated in the regulation of susceptibility to autoimmune and inflammatory conditions, and could play a role in the pathogenesis of blood pressure and type (2) diabetes mellitus, so this study was conducted toevaluate the effect of some demographical parametersand to assess the gene expression levels of VDR gene in both type (2) diabetic and hypertensive patient groups in comparison with apparently healthy controls.A total of (104) individuals were enrolled in this study ,forty of diabetes mellitus type 2 patients, forty of blood hypertension patients and twenty four of apparently healthy individuals as a control group. The age, gender, living region and family historywere studied.Real time – quantitative polymerase chain reaction (RT-qPCR) technique was adopted to assess the amplification levels of VDR gene. The results showed thatthere were significant associations between patients groups and gender and family history, respectively, and there was no significant association (p ≥ 0.05) for the living region in diabetic patients.However, a significant positive association (p ≤ 0.05) exists between living region and Diabetic condition. RT-qPCR amplification results showed that the mean ± SD of threshold cycle (Ct) values of amplification cycles of diabetic, hypertension, and control groups were 23.5±1.22, 24.67±1.30 and27.41±1.94 respectively. The statistical analyses of quantitative amplifications revealed that there was highly significant difference (p ≤ 0.01) between diabetic and control groups, and there were no statistically differences (p ≥ 0.05) of hypertension patients in comparison with control group. The results also revealed significant difference (p ≤ 0.05) between diabetic and hypertensive groups. | ||
| Keywords | ||
| VDR gene; PCR; Diabetes; Hypertension; VDR | ||
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