Added Sheep Serum to Maturation Media to Study the Effects on Maturation, Fertilization and Early Embryonic Development for Locale Sheep in Vitro

	Added Sheep Serum to Maturation Media to Study the Effects on Maturation, Fertilization and Early Embryonic Development for Locale Sheep in Vitro
journal of the college of basic education
Article 1, Volume 12, Issue 56, 2009, Pages 653-660
Author
Mohammed A. Karim AL-Taaie
Abstract
The aim of this study is to determine the important of addition sheep serum to maturation media and know the ability of oocyte for maturation , fertilization and early embryonic development , and determent the ability of surround oocyte by many layer of cumulus cells or denuded on maturation , fertilization and early embryonic development , we divided the oocytes for three group (A ,B and C) , A which present the surround oocyte by 3 – 6 layer of cumulus cell , group B 1 – 2 from cumulus cell and group C denuded ( no layer of cumulus surrounded ) , we used tissue culture media -199 ( TCM-199) after added sheep serum in three level ( 0 , 5 and 10 % ) , we found significant differences (P<0.05) among three groups of oocyte in group A we see higher percent of maturation , fertilization and early embryonic development at level 10% from sheep serum 69.44 , 32 and 25 % respectively , but the oocyte in group C gave low ability in maturation and fertilization 27.9 and 8.33 % in media which added 10 % from sheep serum ,although the result declared that did not added sheep serum to maturation media (level 0 ) decreased the maturation percent 46.6 , 36.11 and 6.45 % for three group A , B and C respectively , within failed oocyte maturation in vitro fertilization

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