Evaluation of Candida albicans attachment to flexible denture base material (valplast) and heat cure acrylic resin using different finishing and polishing techniques

HM, Amir; MJ, Mustafa

	Evaluation of Candida albicans attachment to flexible denture base material (valplast) and heat cure acrylic resin using different finishing and polishing techniques
Journal of baghdad college of dentistry
Article 1, Volume 23, Issue 4, 2011, Pages 36-41
Authors
Amir HM; Mustafa MJ
Abstract
ABSTRACTBackground: Surface roughness of the denture play a major role in the adhesion of microorganisms, it affectedprimarily by the finishing and polishing techniques, the aim of this study was to evaluate the effect of two finishingand four polishing techniques on the surface roughness of flexible denture base (Valplast)and heat cure acrylic andits effect on Candida albicans attachment with the effect of saliva on Candidal adhesion .Material and method: Three hundred twenty square samples (2cm2cm3mm) were prepared (160 flexible acrylicand 160 heat cure acrylic as control), the flexible acrylic and heat cure samples were divided into two major groupsaccording to the finishing method. Group A finished by stone bur, tungsten carbide bur and sand paper).Group B finished by special green cone (silicone finisher bur). Each group divided to four subgroups according tothe polishing method, subgroup contains (20 samples flexible acrylic, 20samples heat cure acrylic). The mean surfaceroughness values (Ra)were gained for each sample after finishing and polishing, the samples then incubated inmedia containing suspension of Candidal cell(conc.of 1*10^6cell/ml)for sixty minutes at room temp ,after that theywere dried, fixed and stained. Ten samples of each subgroup were incubated in artificial saliva for thirty minutesbefore adherent assay.Result: Statistical analysis revealed that flexible acrylic samples that were finished by special green cone with thedifferent polishing techniques were smoother in profilometric study with significance difference in Candidalattachment.Conclusion: Using of special green cone with different polishing techniques for flexible acrylic and heat cure acrylicwill yield smoother surface more than using stone bur, tungsten carbide bur and sand paper, Candida albicansattachment affected by the finishing and polishing method, the method that yield less surface roughness valueshave less Candidal attachment, saliva decreases the attachment of C. albicans.Key Word: Valplast poly(methylmethacrylate),Candida albicans, Finishing ,Polishing, Saliva. (J Bagh Coll Dentistry2011;23(4):36-41).

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