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Effect ofDifferent CellSuspension Culture Densitiesof Physalisangulata L. by Using Plating or Embedding Methods on the Callus Formation | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 12, Issue 1, 2018, Pages 25-32 | ||
| Authors | ||
| MuthanaMuhamad Al-Mahdawe; TalfanAnad Ahmad; Dhuha Sabah Nadir | ||
| Abstract | ||
| The study was successful in establishing cell suspensions cultures derived from the hypocotylcallus of the plant Physalisangulata L. induced in the solid MS medium supplemented with the concentration of 3.0 mg. L-1 2,4-Dichlorophenoxy acetic acid (2,4-D)plus 0.5 mg. L-1 Kinetin(Kin). The culture of the suspensions by different densities (12.36, 13.70, 14.75, 15.98)× 105 cell.cm3 using the platingand embeddingmethods by agar layerin the solidMurashige and Skoogmedium (MS) enriched with the same callus induction concentrations, showed that the plating method exceed theembedding method in the number of cellular colonies which reached the average of 79.4 and 15.0 colony.dish-1 at the culturing density of 15.98 × 105 cell.cm3, these values were declined with the primary density to reach the average of 14.0 and 11.4 colony.dish-1by both the plating and the embedding methods respectively, the high density significantly exceed all the rest densities and gave rate of callusprimordia of 56.6 primordia.dish-1 after 28 days from the day of culturing the suspended cells by plating method, and reached 9.8 primordia.dish-1 after 33 days from the day of culturing of the suspended cells by using the embeddingmethod, the transfer of the callus primordia to the MS medium supplemented with 3.0 mg. L-1 2,4-D plus 0.5 mg. L-1 Kin, led to the growth of callus segments and their differentiation to the somatic embryos and their development through all their stages until reaching the shoot formation. | ||
| Keywords | ||
| Physalisangulata; cell suspension; callus | ||
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