Identification of salmonella typhi by serological and molecular tests isolated from blood

Abod Farhan, Abbas; Shamkhi Jebur, Mohammed; Ayad Abbas, Ruqia

	Identification of salmonella typhi by serological and molecular tests isolated from blood
Diyala Journal of Medicine
Article 1, Volume 14, Issue 2, 2018, Pages 127-137
Authors
Abbas Abod Farhan; Mohammed Shamkhi Jebur; Ruqia Ayad Abbas
Abstract
Background:Salmonella Typhoid Diagnosis by Widal Test and Polymerase Chain Reaction (PCR). Objective:To study the Isolation and diagnosis of Salmonella typhi,diagnosis of the use of the widal test , diagnosis of Salmonella typhi) using PCR technique and Detection of Salmonella typhi. Patients and Methods:The study included the collection of 120 blood samples, with 59.16% (71) and female patients (40.83%), 49 years of age (60-1 years), 24.16% of patients in hospital and 75.83% Patients who are not in hospital. Results:The samples were initially identified using the Widal test as a traditional method of diagnosis and the rate was 75 positive and 45 negative, 20 blood samples were isolated from the suspected disease with typhoid fever, and the diagnosis of bacterial isolates was 17 Widal test (85% (P> 0.01) The isolates studied under the Polymerase Chain Reaction were detected as carriers of the Flic gene and 16.7%. Based on the emergence of a 599 bp package, a base pair of Nasted PCR was the size of 360bp base pair in the gel A significant difference was found (P> 0.01). Conclusion:Serological methods have been shown to be less effective in laboratory diagnosis to diagnose typhoid fever. The molecular diagnosis of PCR can be relied upon as a more accurate diagnosis of typhoid fever infection than the bacterial isolates that own a Flic gene.
Keywords
Salmonella typhi; Salmonella enteric serover Typhi; Widal test; Flic gene; Flic

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