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Treatment of Waste Paper Using Ultrasound and Sodium Hydroxide for Bioethanol Production | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 12, Issue 1, 2018, Pages 108-114 | ||
| Authors | ||
| Osama F. Saeed; Rafid A. Abdulkareem; Shatha K. Muallah | ||
| Abstract | ||
| Bioethanol produced from lignocellulose feedstock is a renewable substitute to declining fossil fuels. Pretreatment using ultrasound assisted alkaline was investigated to enhance the enzyme digestibility of waste paper. The pretreatment was conducted over a wide range of conditions including waste paper concentrations of 1-5%, reaction time of 10-30 min and temperatures of 30-70°C. The optimum conditions were 4 % substrate loading with 25 min treatment time at 60°C where maximum reducing sugar obtained was 1.89 g/L. Hydrolysis process was conducted with a crude cellulolytic enzymes produced by Cellulomonas uda (PTCC 1259).The maximum amount of sugar released and hydrolysis efficiency were 20.92 g/L and 78.4 %, respectively. Sugars released from waste paper were fermented into bioethanol with Saccharomyces cerevisiae. The maximum concentration of bioethanol estimated was 9.5 g/L after 48h of cultivation, the yield and volumetric productivity were 0.454 g/g glucose and 0.2g bioethanol/ L h. respectively. This study of ultrasound and sodium hydroxide treatment may be (we think) it will be a promising technique to develop bioethanol production from waste paper. | ||
| Keywords | ||
| Bioethanol; Cellulomonas uda; ultrasound; waste paper | ||
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