Effect of aqueous extract of Rosemary officinalis on cytotoxicity of  CCL4 induced albino male mice

Mohammed Ibrahim, Ruqaya

	Effect of aqueous extract of Rosemary officinalis on cytotoxicity of CCL4 induced albino male mice
Jornal of Biotechnology Research Center
Article 1, Volume 12, Issue 1, 2018, Pages 124-131
Author
Ruqaya Mohammed Ibrahim
Abstract
This study focused the line on the effect of aqueous extract of Rosemary officinalis, as well as, effect of toxic compound CCL4, on micronucleus formation and mitotic index assay in albino male mice. This work started at September 2017 at Biotechnology Research center Al-Nahrain University, by using 20 albino male mice. The result indicated that aqueous extract of rosemary caused significant increased in mitotic index and decrease micronucleus formation for two doses tested 50,100 mg/kg in comparison with negative and positive controls, also the results revealed that CCL4 showed significant mutagenic action on biological system of treated mice by increased frequency of micronucleus formation and decreased the percentage of mitotic index in bone marrow cells. Pre-and post –treatment between aqueous extract and CCL4 were also made. The results of pre and post treatment with rosemary extract were also caused a significant decreased in micronucleus formation and increase the percentage of mitotic index for two doses 50,100 mg/kg in comparison with its corresponding controls which caused increased in the frequencies of micronucleus formation and decrease the percentage of mitotic index in bone marrow cells. Conclusions: Rosemary officinalis enhanced immunity, reduced mutagenic effects against cytotoxicity of CCL4.
Keywords
rosemary officinalis; CCL; Mitotic Index; Micronucleus percentage

Statistics Article View: 78 PDF Download: 22