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Isolation and identification of bacteria in dummy sucking children with gastroenteritis (Comparative study) | ||
| Journal of baghdad college of dentistry | ||
| Article 1, Volume 22, Issue 4, 2010, Pages 111-114 | ||
| Authors | ||
| Mohammed I. Abud Al-Shaheed; Esra H. Al- Hashemi | ||
| Abstract | ||
| ABSTRACTBackground: Dummy sucking had been associated with many adverse effects on general health of the children, oneof them is infection due to contaminations, this study included the isolation and identification of microorganismassociated with gastroenteritis dummy sucking infected children to determine the most common microorganismisolated in the sample of study group with age ranged between (1 -36) months from both gender in AL-karamaTeaching Hospital between Sep. 2008 to Jan. 2009.Material and methods: the total studied specimens were sixty five (65) dummy swabs, Forty five dummy swabs fromgastroenteritis infected children (group I) and twenty dummy swabs from children without gastroenteritis (group II).The samples were collected with sterile cotton swab and processed for direct microscopic examination (gram stain,wet stain) then used culture for isolation and identification of microorganisms (swabs streaked on blood agar,macConky agar and choclate agar). The morphology of different types of colonies was recorded and smears ofthese different colonies were done by study the Gram‘s stain reaction, microscopic characteristics, colonymorphology on growth media then different types of colonies were subculture and stored for further biochemicaltests to reach complete identification of each isolates, in addition some isolated of gram negative were diagnosedby API 20 E ® system (trade mark of commercial strips for identification of gram negative rods).Results: Ninety two bacterial isolated were obtained in this study, sixty four from group I and twenty eight from groupII. The results revealed that there is statistical significant difference (P < 0.05) between the two groups concerningE.coli and Klebsiella species which is the most common bacteria isolated from group I, while the most commonbacteria isolated from group II was Staphylococcus epidermidis.Conclusion: There is significant appearance of E.coli and Klebsiella species in dummy sucking gastroenteritis infectedchildren and suggested that the use of a pacifier is significant risk factor for recurrent of gastroenteritis .Keywords: Dummy sucking, gastroenteritis. (J Bagh Coll Dentistry 2010;22(4):111-114). | ||
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