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Evaluation of the antimicrobial effects of ozonated water on the sanitization of endodontic files contaminated with C. Albicans | ||
| Sulaimani Dental Journal | ||
| Article 3, Volume 2, Issue 2, 2015, Pages 72-76 PDF (400.27 K) | ||
| DOI: 10.17656/sdj.10042 | ||
| Authors | ||
| Niaz H. Ghareeb H. Saeed1; Chiman H. Fattah2; Kawa A. M. Amin2; Shakhawan Kadir Kadir2 | ||
| 1College of Dentistry, Hawler Medical University. | ||
| 2College of Medicine, University of Sulaimani. | ||
| Abstract | ||
| Objectives: The aim of this study was to evaluate the effectiveness of 0.5 ppm ozonated water in the elimination of Candida albicans, from endodontic files. Materials and Methods: The study was performed on 20 K-files, 21 mm long and of size 25. Of these, five files were taken as a control group, and the remaining 15 files were divided into three groups of 5 files each and they were tested for the efficacy of sanitization with different disinfecting material: sodium hypochlorite alone, sodium hypochlorite followed by ozonated water and ozonated water alone. From the prepared sample of C.albicans, the 20 K-files were contaminated. Results: Antimicrobial effectiveness was evaluated by the reduction of microbial counts. Incomplete disinfection of ozonated water up to 20% was observed (%80 sterilization). Conclusions: The results showed that disinfecting and exposing to ozonated water alone cannot give complete sterilization. Sodium hypochlorite followed by ozonated water can be used as an alternative method for using ozonated water alone. | ||
| Keywords | ||
| Ozonated water; endodontic files; candida albicans | ||
| References | ||
|
1. Sundquvist G. Ecology of the root canal flora. J Endod 1992; 18:427-30.
2. Miller CH. Infection control and office safety sterilization: Disciplined microbial control. Dental Clinics of North America 1991;35:339-55.
3. Rajkumar K, Lakshminarayanan L. The Effectiveness of two commonly used methods of Sterilizing Endodontics. JIDA 2001;72:245-8.
4. Nagayoshi M, Kitamura C, Fukuizumi T, Nishihara T, Terashita M. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod 2004;30:778-81.
5. Kim JG, Yousef AE, Dave S. Application of ozone for enhancing the microbiological safety and quality of foods: a review. J Food Prot. 1999;62:1071-87.
6. Nagayoshi M, Fukuizumi T, Kitamura C, Yano J,
Terashita M, Nishihara T. Efficacy of ozone on survival and permeability of oral microorganisms. Oral Microbiol Immunol. 2004;19:240-6.
7. Thaman D, Sood P. Ozone Therapy in Conservative Dentistry and Endodontics: An Overview .Indian J Stomatol 2012; 3(3):165-69.
8. Guinesi SA ,Andolfatto A ,Bonetti Filho I ,Cardoso AA ,Passaretti Filho J ,Farac RV. Ozonized Oils: A
Qualitative and Quantitative Analysis. Braz Dent J 2011; 22(1): 37-40.
9. Rothchild JA, Harris R.Emollica, PJ. Current Concepts of Oxygen Ozone Therapy for Dentistry in the United States .Int J Ozone Therapy 2010; 9: 105-108.
10. Bo D, Di Z, Jian-ping W. Scanning Electron Microscopic Analysis of Smear Layer Removal in Root Canals by ozonated Water [Abstract]. Shanghai Kou Qiang Yi Xue 2009; 6.
11. Baysan A, Whiley RA, Lynch E. Antimicrobial effect of a novel ozone- generating device on micro-organisms associated with primary root carious lesions in vitro. Caries Res. 2000;34(6):498–501.
12. Baysan A, Lynch E. Effect of ozone on the oral microbiota and clinical severity of primary root caries. Am J Dent. 2004;17(1):56–60.
13. Huth KC, Jakob FM, Saugel B, Cappello C, Paschos E, Hollweck R, et al. Effect of ozone on oral cells compared with established antimicrobials. Eur J Oral Sci. 2006;114(5):435–40.
14. Hems RS, Gulabivala K, Ng YL, Ready D, Spratt DA. An in vitro evaluation of the ability of ozone to kill a strain of Enterococcus faecalis. Int Endod J. 2005;38(1):22–9.
15. Mohammed N. A. 2008 .Detection of Candida spp. and other pathogens responsible for vulvovaginitis in women with contraceptive methods. M.Sc. Thesis. University of Baghdad.
16. Fattah H. Chiman.2013.Detection and characterization of Candida spp. From oral cavity in Leukemic patients from Sulaimani Governorate, Kurdistan Region –Iraq. M.Sc. Thesis. University of Sulaimani.
17. Tan G.L; and Peterson E.M. 2005. CHROMagar
Candida Medium for Direct Susceptibility Testing of Yeast from Blood Cultures. Journal of clinical Microbiology; 43(4): 1727–1731.
18. Byadarahally Raju S, Rajappa S. Isolation and identification of Candida from the Oral Cavity. ISRN Dentistry 2011; 2011, Article ID 487921, 7 pages.
19. Sanjeev Kumar, AnamikaVyas, Mrityunjay Kumar, S.K. Mehra .Application of CHROMagar Candida for Identification of Clinically Important Candida Species and their Antifungal Susceptibility Pattern.Int J Biol Med Res. 2013; 4(4): 3600-6
20. Janga RK, Sudhana M, Tummala H. Comparative
Evaluation of Antimicrobial Efficacy of Sodium Hypochlorite and Ozone Gas and Ozone Water as Irrigants on Enterococcus faecalis An In-Vitro Study. Int J dent clinics 2011:3(3):27-30.
21. De Faria E, Ueno M, Koga-Ito CY, Urruchi WI, Balducci I, Jorge AOC. Effects of ozonated Water on Candida albicans Oral Isolates. Braz J Oral Sci 2005; 4(14):783-786.
22. Pattanaik B,Jetwa D,Pattanaik S,Manglekar S,Naitam DN,Dani A. Ozone Therapy in Dentistry :A Literature Review. J Interdisciplinary Dent 2011; 1(2):87-92.
23. Magni E, Hickel R, Ilie N. Influence of Gasiform Ozone on the Micromechanical Properties of Dentin. Int dent South Africa 2009; 11(2):16-21.
24. Walton RE and Torabinijad M. Principles and Practice of Endodontic.3rdedition.USA: W.B. Saunders company ; 2002 .p. 166,206-238.
25. Baumgartner JC, Cuenin PR. Efficacy of several concentrations of sodium hypochlorite for root canal irrigation. J Endod. 1992;18(12):605–12.
26. Berber VB, Gomes BP, Sena NT, Vianna ME, Ferraz CC, Zaia AA, et al. Efficacy of various concentrations of NaOCl and instrumentation techniques in reducing Enterococcus faecalis within root canals and dentinal tubules. Int Endod J. 2006;39(1):10–7.
27. Zan R, Hubbezoglu I, Sumer Z, Tunc T, Tanalp J.
Antibacterial effects of two different types of laser and aqueous ozone against Enterococcus faecalis in root canals. Photomed Laser Surg. 2013;31(4):150–4.
28. Cardoso MG, de Oliveira LD, Koga-Ito CY, Jorge AO. Effectiveness of ozonated water on Candida albicans, Enterococcus faecalis, and endotoxins in root canals. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;105:85-91
29. Arita M, Nagayoshi M, Fukuizumi T, Okinaga T, Masumi S, Morikawa M, et al. Microbicidal efficacy of ozonated water against Candida albicans adhering to acrylic denture plates. Oral Microbiol Immunol. 2005;20:206-10. | ||
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