Spectrophotometric Methods for Determination of Metronidazole in Pharmaceutical Formulations

Hasan, Shlair H.; Abdullah, Narmeen S.

doi:10.21271/ZJPAS.30.5.7

	Spectrophotometric Methods for Determination of Metronidazole in Pharmaceutical Formulations
ZANCO Journal of Pure and Applied Sciences
Article 7, Volume 30, Issue 5, 2018, Pages 89-100 PDF (755.16 K)
DOI: 10.21271/ZJPAS.30.5.7
Authors
Shlair H. Hasan¹; Narmeen S. Abdullah²
¹Department of Food technology, College of Agriculture, University of Salahaddin, Erbil, Iraq.
²Department of Chemistry, College of Science, University of Salahaddin, Erbil, Iraq
Abstract
Two new, simple and accurate spectrophotometric methods for determination of metronidazole (MET) have been proposed. The methods are based on the reduction of metronidazole with iron metal and hydrochloric acid then the subsequent of the formed reduction product which contains primary amino (NH2) group coupling with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) reagent to form a purple colored chromogen that measured spectrophotometrically at 546 nm (Method A). The method B depends on the reduction of tungstate and/or molybdate in Folin-Ciocaleteu reagent (FCR) by reduced metronidazole in alkaline medium to form a blue colored chromogen, which measured spectrophotometrically at 725 nm. Under optimized reaction condition, the reaction of reduced metronidazole with both NBD-Cl and FCR get a good linearity in the concentration range of 0.3-30 and 0.5-40 μg/ ml for NBD-Cl and FCR, respectively. The limits of detection and quantitation were 0.16 and 0.56 μg/ml for method A; while for method B were 0.05 and 0.18 μg/ml, respectively. The accuracy and precision of the proposed methods were satisfactory. The proposed methods were simple, rapid and successfully applied for analysis of MET in its pharmaceutical formulations.
Keywords
Metronidazole; spectrophotometry; NBD-Cl; FCR; Pharmaceutical Formulations

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