Effect of mercury on the total and electrophoretic profile of proteins and on the activity of three enzymes in kidneys of three species of freshwater clam : Unio tigridis, Pseudontopsis euphraticus and Anodonta sp

M. Mohammed, Solaf; A.Thaker, Abed

doi:10.37652/juaps.2008.15289

	Effect of mercury on the total and electrophoretic profile of proteins and on the activity of three enzymes in kidneys of three species of freshwater clam : Unio tigridis, Pseudontopsis euphraticus and Anodonta sp
Journal of University of Anbar for Pure Science
Article 6, Volume 2, Issue 2, 2008, Pages 24-35 PDF (487.68 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2008.15289
Authors
Solaf M. Mohammed¹; Abed A.Thaker^* ²
¹College of Science - Sulaymaniyah University
²College of Science - Anbar University
Abstract
A three species of clam Unio tigridis, Pseudontopsis euphraticus and Anodonta sp. were collected from Al-Habaniya lake, Al-Angor region/ Al-Anbar- Iraq .The animals were exposed to three concentrations of mercury :0.1, 0.2 and 0.4 mg/l . First group of animals were exposed for three weeks ,second group for two weeks and the third group for one week. At the end of the exposure period, the animals were dissected ,then the kidney removed for the further study on proteins and enzymes. The results were : 1. Total protein increased in the kidney of the third species at all mercury concentrations and decreased in the kidney of the first and second species . We observed prominent effect of mercury on the electrophoretic bands of proteins with the decrease in the intensity of protein bands or induction of new bands. 2. The activity of the three enzymes : Alkaline phosphatase , GPT and GOT were not stable, either increasing or decreasing according to the different species and concentration of the mercury , for example the activity of alkaline phosphatase increased in the kidney of the first species at lower and decreased at the two other concentration . The activity of GPT increased in the kidney of the first species at all concentrations of mercuy, while the activity of GOT decreased at first concentration in the kidnies the first and third species

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