PREPARATION AND ROLE OF BIOPHOS IN NUTRINT AND RESISTANCE Citrullus vulgaris L. TO THE INFECTION OF Fusarium wilt d.

A.Sabee, A; F.Alrawi, D.; A.Assaffii, A.

doi:10.37652/juaps.2007.15374

	PREPARATION AND ROLE OF BIOPHOS IN NUTRINT AND RESISTANCE Citrullus vulgaris L. TO THE INFECTION OF Fusarium wilt d.
Journal of University of Anbar for Pure Science
Article 14, Volume 1, Issue 2, 2007, Pages 64-74 PDF (550.77 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2007.15374
Authors
A A.Sabee¹; D. F.Alrawi²; A. A.Assaffii³
¹College of Science - University of Diyala
²College of Education - Anbar University
³College of Science - Anbar University
Abstract
Lab. Experiments to prepare Biophos has been carried out. The experiments was based on a field observation for three seasons at the area Zankoria west Ramadi city 25 km Anbar governorate, Iraq. From field observation and Lab analysis, we have F.oxysporum that infected plant and cause fusarium wilt disease, and two isolate Streptomycets sp.f3-y3 , Streptomycets sp.f3-y3.that had ability to inhibitor F.oxysporum . A clay soil and plants powder (Cyperus rotundus Linn., Phragites austrialis ,Eucalyptus microtheca ) were used in percentage 20,10,40,30% respectively. The prepare compos were mixed up with powder of rock phosphate –appetite with compos in ratio 1:1,0.5:1, 0:1.The mixture was wet with sterilization whey and then it was inoculated with St.ts sp.f3-y3., P. fluorescence f1-y1, that has been isolated from the observation aria (as to be at the highest total density microbes on the roots of un affected plants). The treatments was incubated in 28± 2 c؛ for 20,40,60 days. Some of lab. Test were carried out analysis had been carried out on the results of the lab. test. The results indicated that prepared material for the Biophos - St sp.f3-y3 for an incubated period 60 days, was recorded the highest contents of phosphorus, nitrogen, humic and falvic acids in additions biomass, the rafter the treatment of the prepare material with inoculated P. fluorescence f1-y1 for an incubated period 40 days. The ability of the prepared material and their extract 5:1 w.d. Ratio. Were tested in the inhibition of the F.oxysporum. The result indicated the activity of the extracted the mixture of two materials Biophos and the material mixture as the give the highest ability for completely inhibition F.oxysporum. Both characterizing material were used to prepare treatment from Biophos St. sp.f3-y3, Biophos-P. fluorescence f1-y1 with a ratio of 1:1. The treatment were adding in an amount of 20 g. / plant by using two different methods-with seeds 2-partitioning the quantity on two share, first gave with seeds, while the other after a month of plantation-adding after one month of plantation. Another treatment was carried out by used mixture from two isolates inoculums were added with like method. Inoculums of F.oxysporum uses with treatments or without it (control treatment). The treatments were carried out in the same field area which planted with watermelon (Charleston Negara Citrullus vulgaris L) the percentage of infected and average productions were recorded. The result revealed the superiority of the mixture that parting in two shares, to given the lowered infection percentage 8.5% and highest productivity 40 ton/ha
Keywords
Bio; phos

References
[1] Biophos, conitued .Copyright C. Sinclair 2002, Edition 1. 06/03/2002. [2] Peter A. Vandenbergh, Carlos F. Gonzalez, Ann M. Wright, and Blair S. Kunka.1983.Iron-Chelating Compounds Produced by Soil Pseudomonads: Correlation with Fungal Growth Inhibition. Appl Environ Microbiol. 1983 July; 46(1): 128–132. [3] Saikia R, Singh T, Kumar R, Srivastava J, Srivastava AK, Singh K, Arora DK.2003.Role of salicylic acid in systemic resistance induced by Pseudomonas fluorescens against Fusarium oxysporum f. sp. ciceri in chickpea. Microbiol Res. 2003; 158(3):203-13. [4] Reports on plant Diseases.1983. RPD No.904- Fusarium Wilt of Watermelon and Muskmelon. [5] Zhou,X.G. and Everts,K.L.2004. Quantification of root and stem colonization of watermelon by Fusarium oxysporum f.sp.niveum and its use in evaluating resistance. American phytpathological Soc.94.8.832-841. [6] Crawford, D.L.; Lynch, J.M.; Whipps, J. M. and Ousley, M.A. 1993.Isolation and characterization of actenomycete antagonists of a fungal root pathogen. Applied and Environmental Microbiology 59.3899-3905. [7] Franco, M. and Valenica, H. 2005. Evaluation of actenomycetes as growth inhibitor of Fusarium oxysporum f. sp dianthi in carnations. Abstract. Marcela, Franco corlea 7^a No.43-82 [8] Lixiang Cao, Zhiqi Qiu, Xin Dai, H ONGMING Tan, Yongcheng Lin and Shining Zhou.2004. Isolation of endophytic actinomycetes from roots and leaves of banana plants and their activities against Fusarium oxysporum f. sp cubense.World J. of Microbiology and Biotechnology 20; 501-504. [9] Aghighi, S.; Shahidi Bonjar, G.H; Rawashdeh, R.; Batayneh, S. and Saadoum,I.2004.First report of antifungal spectra of activity of Iranian actenomycetes straians against Alternria solani , Alternaria alternate , Fusarium solania, Phytophthora megasperma,Verticillium dahliae and Saccharomyces cerevisiae.Asian J. of Plant Sciences 3;463-471. [10] Tulemisova KA, Chormonova NT.1989. Search for actinomycetes--antagonists of fungi causing sugar beet root rot. Antibiot Khimioter. 1989 Nov; 34(11):816-9. [11] Pagne, W. J. 1970. Yield and growth of heterotrophs. Ann. Rev. Microbial. 24:17. [12] Cox,C.D. and Graham, R. 1979. Isolation of ionbinding compound from Pseudomonas aeruginosa. J. Bacteriol. 137; 357-364. [13] Program static GEN532-2. [14] Hayes, M. H.; Swift, R.S.; Wardle, R.E. and Brown, J. K. 1975. Humic material from an organic soil . Geoderma 13; 231-245. [15] Louw, H.A.; Webley, D.M.A. 1958. Plate method for estimating the number of phosphate devolving and acid productivity bacteria in soil. Natur. Lond.; 182;1317-1318.
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