<i>In Vivo</i> evaluation of the novel nanocomposite porous 3D scaffold in a rabbit model: hematology and biochemistry analysis

Mahmood, S. J.; Zakaria, Md. Z.; Abdul Razak, I. Sh.; Yusof, L. M.; Abubakar, A. A.; Mahmood, Z. K.; Bin Ab Latip, M. Q.

doi:10.33899/ijvs.2019.153853

	In Vivo evaluation of the novel nanocomposite porous 3D scaffold in a rabbit model: hematology and biochemistry analysis
Iraqi Journal of Veterinary Sciences
Article 16, Volume 32, Issue 2, 2018, Pages 219-230 PDF (506.18 K)
Document Type: Research Paper
DOI: 10.33899/ijvs.2019.153853
Authors
S. J. Mahmood^* ¹; Md. Z. Zakaria²; I. Sh. Abdul Razak³; L. M. Yusof⁴; A. A. Abubakar⁵; Z. K. Mahmood⁶; M. Q. Bin Ab Latip⁷
¹Department of Veterinary Anatomy, College of Veterinary Medicine, University of Mosul, Mosul, Iraq
²Laboratory of Molecular Biomedicine, Institute of Biosciences Universiti Putra Malaysia (UPM), 43400,Serdang, Selangor Darul Ehsan, Malaysia
³Laboratory of Molecular Biomedicine, Institute of Biosciences Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400,Serdang, Selangor Darul Ehsan, Malaysia
⁴Department of Companion Animal Medicine and Surgeryو Universiti Putra Malaysia (UPM), 43400,Serdang, Selangor Darul Ehsan, Malaysia
⁵Department of Veterinary Surgery and Radiology, Usmanu Danfodiyo University, Sokoto, Nigeria
⁶Department of Veterinary clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400,Serdang, Selangor Darul Ehsan, Malaysia
⁷Department of Veterinary Preclinical Sciences Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400,Serdang, Selangor Darul Ehsan, Malaysia
Abstract
Issues of safety are very crucial with biomaterials and medical devices. Sixteen male New Zealand White rabbits equally into four groups: Group A, rabbits had part of their radial bone (2 cm, mid shaft) and left empty as a control. Group B, rabbits were implanted with scaffold 5211. Group C, rabbits were implanted with scaffold 5211_GTA+Alginate. Group D, rabbits were implanted with 5211_PLA.All scaffolds were prepared by freeze-drying method. Blood samples were collected at day 0 and 1^st, 2^nd, 3^rd, 4^th and 8^th week after implantation. The blood examination included complete hemogram and certain serum biochemical parameters. The results showed that there was no significant difference (P>0.05) among each treatment group in comparison with control group (day 0). However, red blood cells, hemoglobin, packed cell volume, mean corpuscular hemoglobin concentration, monocyte, plasma protein, inorganic phosphate, sodium, chloride and urea were significantly increased (P<0.05) among treatment groups at week 8. An abnormal architecture of viscera was observed in all animals, thus indicating a form of toxicity related to the degrading scaffold materials. The severity of histopathological lesions in viscera was not coated polymers dependent nor development materials. In conclusion, implantation of 5211 scaffold with or without coated framework has a significant impact on histopathological and certain hematological and biochemical parameters.
Keywords
3D porous scaffolds; Bionanocomposite; coated; biochemical parameters; Rabbit

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In Vivo evaluation of the novel nanocomposite porous 3D scaffold in a rabbit model: hematology and biochemistry analysis