Mohammed Jihad, R. (2012). Simultaneous determination of tryptophan and tyrosine in binary mixture by zero-crossing second derivative spectrophotometry.. , 4(1), 35-39. doi: 10.37652/juaps.2010.15419
Riyadh Mohammed Jihad. "Simultaneous determination of tryptophan and tyrosine in binary mixture by zero-crossing second derivative spectrophotometry.". , 4, 1, 2012, 35-39. doi: 10.37652/juaps.2010.15419
Mohammed Jihad, R. (2012). 'Simultaneous determination of tryptophan and tyrosine in binary mixture by zero-crossing second derivative spectrophotometry.', , 4(1), pp. 35-39. doi: 10.37652/juaps.2010.15419
Mohammed Jihad, R. Simultaneous determination of tryptophan and tyrosine in binary mixture by zero-crossing second derivative spectrophotometry.. , 2012; 4(1): 35-39. doi: 10.37652/juaps.2010.15419

Simultaneous determination of tryptophan and tyrosine in binary mixture by zero-crossing second derivative spectrophotometry.

Journal of University of Anbar for Pure Science
Article 12, Volume 4, Issue 1, 2010, Pages 35-39    PDF (336.13 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2010.15419
Author
Riyadh Mohammed Jihad*
Women Education College, Al-Anbar University
Abstract
Rapid and accurate binary mixture resolution of tryptophan and tyrosine was performed. Differential-derivative spectrophotometry with a zero-crossing measurement technique was used for the quantitative determination of tryptophan and tyrosine in laboratory-prepared mixtures. Neither sample pretreatment nor separation were required. Linear calibration graphs of differential second derivative values were (at 222.4 and 217.9 nm for tryptophan and tyrosine , respectively) versus concentration in the ranges 0.1–20.0 and 1.0– 50.0 µg ml_1, and the linearity was satisfactory (r = 0.9987 and r = 0.9997), for tryptophan and tyrosine , respectively) were obtained .The relative standard deviations were found to be less than 1.03%, indicating reasonable repeatibility of method.The results obtained by the proposed method has been statistically compared by means of Student t-test and by the variance ratio F-test where shows a good agreement .
Keywords
tryptophan; tyrosine; spectrophotometry; Simultaneous determination; zero; Crossing
References
  1. Nelson, D.L.; and Cox, M.M.; (2000). Lehninger Principles of Biochemistry, 834, Worth Publishers, 41 Madison Avenue, Newyork.
  2. Ikeda, M.; (2002). Amino Acid Production Processes. Adv. Biochem. Eng. Biotechnol.;79, 1–35.
  3. Sanchez-Machado, D. I. ; Chavira-Willys, B. and; Lopez-Cervantes, J. ; (2008). High-performance liquid chromatography with fluorescence detection for quantitation of tryptophan and tyrosine in a shrimp waste protein concentrate. J. Chromatogr.; 863, 1, 88–93.
  4. Widner, B.; Werner, E.; Schennach, H.; Wachter, H.; and Fuchs, D.; (1997). Simultaneous measurement of serum tryptophan and kynurenine by HPLC. Clin. Chem.; 43, 2424–2426.
  5. Maneglier, B.; Rogez-Kreuz, C.; Cordonnier, P.; Therond,P.; Advenier,C.; Dormont,D.; Clayette,P.; and Spreux-Varoquaux,O.; (2004). Simultaneous Measurement of Kynurenine and Tryptophan in Human Plasma and Supernatants of Cultured Human Cells by HPLC with Coulometric Detection. Clin. Chem.; 50,11, 2166 – 2168.
  6. Andreas, L.; Gabriele, N.; Bernhard, W.; and Dietmar, F.; (2002). More Rapid   Method for Simultaneous Measurement of Tryptophan and Kynurenine by HPLC. Clin. Chem.; 48,579–581.
  7. Majidi, M.; Jouyban, A.; Abdollahi, H.; and Asadpour-Zeynali, K.; (2006). Simultaneous Voltammetric Determination of Cysteine, Tyrosine and Tryptophan by Using Principal Component-Artificial Neural. Networks. Asia. J.Chem.; 18, 4, 2445–2457.
  8. William, N. ; (1989). Pyrolysis Gas Chromatography of Wool, Part II: Detection and Quantitation of Tryptophan in Wool and Simple Proteins. Text. Res. J.; 59, 10, 616–622.
  9. Anderson, G.; Young, J.; and Cohen, D.; (1979). Rapid liquid chromatographic determination of tryptophan, tyrosine, 5-hydroxyindoleacetic acid and homovanillic acid in cerebrospinal fluid. J. Chromatogr.; 164,4, 501–505.
  10. Baranowska, I.; and Kozowska, M.; (1995). TLC separation and derivative  spectrophotometry of some amino acids. Talanta; 42, 10, 1553–1557.
  11. Bhushan, R.; Ali, I.; and Sharma,S.; (1995). A comparative study of HPLC and TLC separation of amino acids using Cu(II) ion. Biomed. Chromatogr.; 10,1, 37–39.
  12. Guang-Ping , J.; and Xiang-Qin ,L.; (2004). The electrochemical behavior and amperometric determination of tyrosine and tryptophan at a glassy carbon electrode modified with butyrylcholine. Elect .Chem. Commun. ; 6, 5, 454–460.
  13. Lin , X.; Kang, G.; and Zhu, X.; (2008). Uracil Grafted Carbon Electrode: Electrocatalytic Behavior of Tryptophan, Tyrosine, Catecholamine and Related Compounds. Chin. J. Chem.; 26, 4, 681–688.
  14. Mozo-Villarias, A.; (2002). Second derivative fluorescence spectroscopy of tryptophan in proteins. J. Biochem. Biophys. Meth.; 50, 163–178.
  15. Nayar, S.; Brahma, A.; Mukherjee, C.; and Bhattacharyya, D.; (2002). Second derivative fluorescence spectra of indole compounds. J. Biochem.; 131, 427–435.
  16. Luethi-Peng, Q.; and Puhan, Z.; (1999). Determination of protein and casein in milk by  fourth derivative UV spectrophotometry. Anal. Chim. Acta.; 393 , 227–234.
  17. Kumar, V.; Sharma,V.K.; and Kalonia,D.S.; (2005). Second derivative tryptophan fluorescence spectroscopy as a tool to characterize partially unfolded intermediates of proteins. Inter. J. Pharm.; 294 , 193–199.
  18. Talsky,G.; (1994). Derivative Spectrophotometry; Low and High Order, VCH,  Weinheim.
  19. Antonov,L.; (1997). Fourth derivative spectroscopy - a critical view. Anal. Chim. Acta. ; 349 , 295–301.
  20. Griffiths, T.R.; Keith, K.; Hubbard,H.; Schwing-Weill,M.; and Meullemeestre, J.; (1982). Some aspects of the scope and limitations of derivative spectroscopy . Anal. Chim. Acta., 143, 163-176.
  21. Dave,H.N.; Mashru,R.C.; and Thakkar,A.R.; (2007). Simultaneous determination of salbutamol sulphate, bromhexine hydrochloride and etofylline in pharmaceutical formulations with the use of four rapid derivative spectrophotometric methods. Anal. Chim. Acta.; 597, 1, 113–120.
  22. Erk, N.; Ozkan, Y.; Banoglu, E.; Ozkan, S. A.; and Senturk, Z.; (2001). Simultaneous determination of paracetamol and methocarbamol in tablets by ratio spectra derivative spectrophotometry and LC. J. Pharm. Biomed. Anal.; 24, 3, 469–475.
  23. Dinc,E.; (1999). The spectrophotometric multicomponent analysis of a ternary mixture of ascorbic acid, acetylsalicylic acid and paracetamol by the double divisor-ratio spectra derivative and ratio spectra-zero crossing methods. Talanta; 48, 5, 1145–1157.
  24. Morelli,B.; (2003). Determination of binary mixtures of analgesic and spasmolytic drugs in pure and dosage forms by derivative spectrophotometry. J. Pharm. Biomed. Anal.; 33, 3, 423-433.
Miralles, B.; Bartolome, B.; Ramos, M.; and Amigo, L.; (2000). Determination of whey protein to total protein ratio in UHT milk using fourth derivative spectroscopy. Inter. Dair. J.; 10, 191–197.

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