J. MOHAMED, M., SALMAN, S., M. AL-ALANI, L., T. ABED, S., D. ABOD, A., A. EBRAHEIM, M., M. FARHAN, A. (2012). ISOLATION AND IDENTIFICATION OF MICROBIAL FLORA IN EYE AND STUDY THE EFFECT OF EYE LINER AND CONTACT LENSES USING ON THE MICROBIAL FLORA ON IT. , 3(2), 30-32. doi: 10.37652/juaps.2009.15437
MARWA J. MOHAMED; SAMERAM. SALMAN; LAITH M. AL-ALANI; SAMERA T. ABED; AMAL D. ABOD; MUNA A. EBRAHEIM; ANMAR M. FARHAN. "ISOLATION AND IDENTIFICATION OF MICROBIAL FLORA IN EYE AND STUDY THE EFFECT OF EYE LINER AND CONTACT LENSES USING ON THE MICROBIAL FLORA ON IT". , 3, 2, 2012, 30-32. doi: 10.37652/juaps.2009.15437
J. MOHAMED, M., SALMAN, S., M. AL-ALANI, L., T. ABED, S., D. ABOD, A., A. EBRAHEIM, M., M. FARHAN, A. (2012). 'ISOLATION AND IDENTIFICATION OF MICROBIAL FLORA IN EYE AND STUDY THE EFFECT OF EYE LINER AND CONTACT LENSES USING ON THE MICROBIAL FLORA ON IT', , 3(2), pp. 30-32. doi: 10.37652/juaps.2009.15437
J. MOHAMED, M., SALMAN, S., M. AL-ALANI, L., T. ABED, S., D. ABOD, A., A. EBRAHEIM, M., M. FARHAN, A. ISOLATION AND IDENTIFICATION OF MICROBIAL FLORA IN EYE AND STUDY THE EFFECT OF EYE LINER AND CONTACT LENSES USING ON THE MICROBIAL FLORA ON IT. , 2012; 3(2): 30-32. doi: 10.37652/juaps.2009.15437

ISOLATION AND IDENTIFICATION OF MICROBIAL FLORA IN EYE AND STUDY THE EFFECT OF EYE LINER AND CONTACT LENSES USING ON THE MICROBIAL FLORA ON IT

Journal of University of Anbar for Pure Science
Article 8, Volume 3, Issue 2, 2009, Pages 30-32    PDF (581.88 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2009.15437
Authors
MARWA J. MOHAMED1; SAMERAM. SALMAN2; LAITH M. AL-ALANI1; SAMERA T. ABED1; AMAL D. ABOD1; MUNA A. EBRAHEIM1; ANMAR M. FARHAN1
1Anbar University - College of Science
2Ministry of Health - Anbar Health Department
Abstract
Our studying includes culturing of the conjunctival swabs on selective media and
enrichment media (Nutrient agar, Machonky agar and blood agar) from for students of Anbar university.
They are (19 – 26) years old, during the period from 2008 to Feb. 2009. The number of samples are 30
swabs divided taking 10 from female (using contact lens). 10 from female (using the eye liner) and (5
male, 5 female) they were a save as control into three groups (They had never worm contact lenses and
eye liner).
They are very deferent in microbial flora between the wearing contact lenses and who had never. The
microbial flora isolated control group was Staphylococcus epidermidis, S. capitis, S. Xylosus, S. areus.
They were 83.32% and Candida in 16.68%. But the microbial flora (Contamination) in contact lenses
using group was the gram negative bacteria Pseudomonas aeruginosain 16.62%. Aeromonas, Klebsiella,
E.coli in 8.33 for each and gram posative bacteria (S. aureus, S. epidermidis) in 25% and B. heamolytic,
Streptococcus in 8.33 and it is including fungi (Aspirigilus spp., Alternaria and Candida in 8.33 for each.
In the eye liner using group the 60% were negative for laboratory culturing and 40% were positive for
laboratory culturing. They were Staphylococcus Epidermidis and S. aureus. The second line of study
include culture for sensitivity to same intibiotic for bacteria. They are appeared that is the antibiotic
Erethromycin is more effect on gram positive and gram negative bacteria they are (69.5% sensitive) then
Tetracycline in 47.8% and Gentumycin in 52% but the more antibiotic which was the bacteria Resisting
was Penicillin G (just 4.7% sensitive) then Ampicillin in 26.9 and Cefalexin in 35.7% because the fals
using of those drugs from the people.
References
  1. الحدیثی، جمال عبد الرحمن إبراهیم. 2007. إمکانیة استعمال طرائق محورة لتشخیص خمج قرینة العین المستعصی. أطروحة دکتوراه- کلیة العلوم- جامعة الأنبار.
  2. الجبوری، محیمید مد الله. 1990. علم البکتریا الطبیة. وزارة التعلیم العالی والبحث العلمی- جامعة الموصل.
    1. Atlas, R. M.; Parks, L. C. and Brown, A. W. 1995. Laboratory of Experimented Microbiology. Mosby - year Book Inc- Baltimore.
    2. Forbes, B. A.; Sahm, O. F. and Weissfeld, A. S. 1998. In faction of the eyes. ear and sinuses Bialy and Scotts Diagnostic Microbiology. 10th edition Mosby.
    3. Kanski, J., Menon, J. and Bolton, A. 2003. Clinical ophthalmology.15thed., Butterworth Heinemann.
    4. Kwon-chung, K. I. and Bennett, J. E. 1992. Medical mycology. Keratomycosis.3rd  ed., lea and Fibiger. Philadelphia London.
    5. Lewis. R. G. and John. 1986. Outpatient treatment of microbial Corneal Ulcers Arch Ophthalmic. 90:38.
    6. Ramphal, R. M. T.; Niece, M. E. and Polack, F. M. 1981. Adherence of pseudomonas aeruginosa to the injured cornea. Astep in the pathogenesis of corned infection. Ann. Ophthalmic.13: 421-425.
    7. Suzanne, M. J. Fleiszig and Nathen. 1992. Microbial Flora in Eyes of current and former control lens wearers. Corneal Biophysics, Department of optometry. University of Melbourne Parkville 3052. Victoria. Australia.
10.Velpandian, T.; Gupta, S. K.  Y. K. Agarwal. Hc and B. was. N.R. 1999. Comparative studies of pical lomefloxaciu and ciprofloxaciu on ocul pharmacol. Ther. 15(6): 505.

11.Wilson, L. A.; Sezton, R. R. and Raw, J. S. 1996. Fung from the mormal outers ege. Am. J. ophthalmool. 67: 52-56.

12.Erdogan. Haydar M. D.; et al. 2002. Effect of freqvent-Replacement contact lenses on normal conjunctive flora. CLAO J., 28 (2):99-95.

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