STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY

.F.AL-RAWI, KHALID

doi:10.37652/juaps.2007.15558

	STUDYING THE EFFECTS OF SOME COMPOUNDS AND IONS ON Escherichia coli ALKALINE PHOSPHATASE ACTIVITY
Journal of University of Anbar for Pure Science
Article 15, Volume 1, Issue 3, 2007, Pages 58-69 PDF (603.86 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2007.15558
Author
KHALID .F.AL-RAWI^*
Department of Chemistry - College of Education - University of Anbar
Abstract
The effect of some compounds and ions such as EDTA,Urea,KCN,2-Mercaptoethanol,BeCl2 and L-Phenyl alanine on the activity of Alkaline phosphatase extracted from Escherichia coli has been studied by using p-NitroPhenyl Phosphate as a substrate at a different concentrations (1.5„e10-1,3.0„e10-2,4.5„e10-3) M.,and the alkaline glycine regulating solution pH=10.5.Inhibition constant Ki and type of inhibition(competitive or noncompetitive)using Dixon plot for competitive & noncompetitive inhibition as well as the percentage of inhibition have all been calculated & I50 for different compounds.
Keywords
compounds; Ions; Escherichia coli; alkaline phosphatase

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