Sh. A Lafi, A., F. Al-Rawii, D., Kh. Al-issa, A., A. Assaffii, I. (2012). Produce the single cell protein (SCP) by the solid culture method from Schanginia aegyptiaca wastes with Aspergillus niger and use in feeding of carp fish (Cyprinus carpi). , 3(1), 23-33. doi: 10.37652/juaps.2009.15563
Ahmed Sh. A Lafi; Daffer F. Al-Rawii; Abdullah Kh. Al-issa; Idham A. Assaffii. "Produce the single cell protein (SCP) by the solid culture method from Schanginia aegyptiaca wastes with Aspergillus niger and use in feeding of carp fish (Cyprinus carpi)". , 3, 1, 2012, 23-33. doi: 10.37652/juaps.2009.15563
Sh. A Lafi, A., F. Al-Rawii, D., Kh. Al-issa, A., A. Assaffii, I. (2012). 'Produce the single cell protein (SCP) by the solid culture method from Schanginia aegyptiaca wastes with Aspergillus niger and use in feeding of carp fish (Cyprinus carpi)', , 3(1), pp. 23-33. doi: 10.37652/juaps.2009.15563
Sh. A Lafi, A., F. Al-Rawii, D., Kh. Al-issa, A., A. Assaffii, I. Produce the single cell protein (SCP) by the solid culture method from Schanginia aegyptiaca wastes with Aspergillus niger and use in feeding of carp fish (Cyprinus carpi). , 2012; 3(1): 23-33. doi: 10.37652/juaps.2009.15563

Produce the single cell protein (SCP) by the solid culture method from Schanginia aegyptiaca wastes with Aspergillus niger and use in feeding of carp fish (Cyprinus carpi)

Journal of University of Anbar for Pure Science
Article 9, Volume 3, Issue 1, 2009, Pages 23-33    PDF (482.76 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2009.15563
Authors
Ahmed Sh. A Lafi1; Daffer F. Al-Rawii1; Abdullah Kh. Al-issa2; Idham A. Assaffii3
1Anbar University/ College of Education for Pure Sciences
2Al-Baath University - College of Agriculture - Syrian Arab Republic
3Anbar University - College of Agriculture
Abstract
The study was conducted to produce the single cell protein and to increase the content of Schanginia
aegyptiaca wastes from the parent microbial protein was to grow isolates from (Aspergillus niger) by the use of nutrient
media of Schanginia aegyptiaca powder and wastes of dairy factories and dates wastes by using incubators and solid
fermentation. The suitable conditions for production such as components media, percentage of support materials, time of
incubation and best isolate for production were determined.
Yield components of single cell protein were checked. The yield, was then used in specified ratios of replacement for the
vegetable protein in the preparation of carp fish (Cyprinus carpi) food. The results were as follows:-
1-Obtaining 10 local isolates inhabiting Schanginia aegyptiaca, three of them were selected depending on the ratio of growth
diameter which ranged between (5.60-6.40) cm on PDA media, The Is-8 isolate has been selected and diagnosed to
belong to fungi A. niger-P8 with A. niger-S isolate.
2-Wetting the powder media of Schanginia aegyptiaca with Whey and extract of dates wastes and fermented in a solid
culture doubled the percentage of the protein in the media up to (5) times. The highest percentage 8.14% was obtained
when wetting withWhey and by the use of A. niger-P8 isolate.
3-The produced protein contained low percentage of nucleic acids RNA and DNA. The totals of their percentage were 5.18%
and 5.32% in liquid culture protein, and 3.10% and 3.72% in solid culture protein for A. niger-P8 and A. niger-S
isolates, respectively, revealed that production are free from toxins.
4- The microbial protein produced contained 15 amino acids. The rates of the amino acids were 64.95 and 63.39 g/100g of
protein, the analysis of the microbial protein showed the highest content of Aspartic; 14.29 and 12.32 g/100g of protein
for A. niger-P8 and A. niger-S isolates, respectively.
5-The percentage of replacement 50% of Soya bean with the microbial protein A. niger-P8 produced gave the highest rate in
the weight of carp Cyprinus carpi 26.22 g per fish after 35 days of feeding with a difference 5.7% compared to control
treatment.
Keywords
Cyprinus carpi; Aspergillus niger
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