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EXPERMENTAL STUDY TO COMPARE BETWEEN THE EFFECT OF ALCOHOLIC AND AQUEOUS EXTRACT OF CLOVE ON THE GROWTH OF STAPHYLOCOCCUS AUREUS ISOLATED FROM MASTITIS IN COWS INVITRO AND INVIVO | ||
| Journal of University of Anbar for Pure Science | ||
| Article 4, Volume 3, Issue 3, 2009, Pages 7-12 PDF (457.77 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2009.15602 | ||
| Authors | ||
| NARJIS .A.ABDUL RAZAAG; HASSAN.A ABDUL-RATHA* | ||
| Baghdad University - College of Veterinary Medicine | ||
| Abstract | ||
| This study was conducted to investigate the role of alcoholic and watery extract of clove on the invitro Inhibition if staphylococcus aureus , which were Isolated from acute bovine mastitis and the therapeutic role of these extract in mammary gland of mice which were used as animal model in experimental infection design. Inorder to study the pathogensis of these Isolates and their role on acut mastitis study included the therapeutic effect of watery extract of clove in the Mammary gland infection compared with the positive group (mice injected withs staph . aureus without treatment) .The watery extract of clove dissolved in water (300) mg /ml showed highly Inhibition effect on stuph . aureus growth in vitro so its gave ( 53.3) mm inhibition zone while the alcoholic extract which dissolved in water showed the lowest effect with (35 ) mm zone by the same concentration at (300)mg/ml . study inclued also the therapeutic effect of daily orally treatment dose 100mg /ml of watery extract of clove in the patgogensis of staph.aureus in these treatment period continous (30) day after 48hr of injection 125cfu/ml in mice in addition to use positive control group (mice injected with staph . aureus with out any treat ment) , clinical signs were shown side effect of clove extract after 15 day of treatment . histological study showed the role of clove extract it’s the same as histopathologcal changes in compared with the negative control and the mammary gland of these animal showed negative growth for staph.aureus . | ||
| Keywords | ||
| Staphylococcus aureus | ||
| References | ||
|
1- Knight, C.(1999) . Oxytocin: an alternative to antibiotic For treating mastitis, Hannuch Research Institute, Year Book.
Fetrow,J .(2000) .mastitis an economic consideration. Inproceding of 2qth annual meeting of Nall. Mast.coun.Allanta,G.A.Nall Mast. Coun. Mwdison,WI., pp:3-47 .
3- Smith, K.L. ; Hillerton,J.E . and Harman , R.J. (2001) .National Mastitis council guide lines on normal and abnomal raw milk based on somatic cell counts and Signs of clinical Mastitis .
4- Murinda, S.E; Ngugen L. T.; Ivey , S . J.; Gillespie, B.E.;Almedia , R.A. and oliver, S.P. (2002a) . Prevalence and molecular characteri zation of Eschericha coli O157: H7 in bulk tank milk and Fecal samples From cull dairy cow;a 12month survey of dairy Farms in East Tennessee .J. Food port.65;752-759 0
5- Elsevier , L.(2007)0 veterinary medicine , text book of the disease of cattle , horses , sheep , pigs and goats , 20th ed.
6- Severin , A., Weiwu ,S. Tabei , K.and Towasz, A. (2005). High level B-lactam Resistance and cell wall synthesis catalyzed by MecA homologue of staphylococcus sciuri Introduction to staph . aureus J.B.vol.187No . 19pp 6651 – 6658.
7- Reugg, P.H. and Tabone , T.J. (2000) . The relationship between antibiotic residue violations and Somatic cell countin wis consindairy herds. J. Dairy Sci. 83: 2805 – 2809 .
8- Fehri , B.; Aiache , J.; Memmi, A.; Korbi, S. and Lamaison , J .(1994) . Hypo tension , Hypo glycemia Hypouricemiai recorded after rpeated administration of equenous leaf extract of Oleauropael. J. Pharm. Belg , 49(2) : 101-101-8.
9- Mahmood , M.J.; Jawed , A.J.; Jawed, A.J.; Hussain , A.M.; Al- omeri, M. and Al-Naib, A.(1989) . Invetro antimicrobial activity of salsola romarinus and Adiantum capillas venris . Int, L. crude . Drug . Res. 27:14-16 .
10- Luna ,L.G.(1968). Manual of Histologic staining methods of armed Forces in stitute of pathology 3rd Ed . Mcgrow Hill book; company . New York .
11- Snedecor , G.W. and cochram, W.G.(1980 ) statiscal methods 7th ed .the lowastate university Press Ames .
12- Grieve , M.(2008) . clove . Botanical Amodern Herbal.
13- Convington , A.D.(1997) . "Modern Tanning chemistry" , J. Chemical Socity Reviews , 26,73-146.
14- مصطفى ، ایمان عبد العزیز .(1995) . التأثیرات البایولوجیة المثبطة لمستخلصات بعض النباتات الطبیة فی بعض الاحیاء الدقیقة المعزولة من قنوات جذور الاسنان . رسالة ماجستیر / کلیة العلوم – جامعة الموصل ، العراق . 15- زنکنة ، شکریة علی محمد . (2004) . تأثیر مستخلصات عدد من النباتات على نمو بعض انواع البکتریة المرضیة . رسالة ماجستیر / کلیة العلوم – جامعة الانبار . 16- Nicolls , C.(2000) . Aromaticine in treatment of infections . British Journal of Phytomedicine, vol.5NO.1.
17- Alfahmi, Z,M, (2007) . The effect of Aloe veral leaf gel in promoting wound healing and as anantibacteracagent AThesis submitted to college of vet . med . university of Baghdad .
18- Barbara McGe, M.G.(2003) encyclopedia of spices (cloves) exclusively Foe the Epicenter .
19- Villepik, R. and Bethesda , MD. (2007) . cloves and clovesoil . Natural stander U.S. National Library of medicin.
20- Bruneton, J. (1995) . Pharmacognosy phytochemistry Plants Itampshier , U.K. Intercept Ltd .
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