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Production of single cell protein from growth local Candida utilis, Candida tropicalis tropicalis Isolates on Whey and remnants of dates | ||
| Journal of University of Anbar for Pure Science | ||
| Article 8, Volume 2, Issue 3, 2008, Pages 30-44 PDF (572.86 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2008.15631 | ||
| Authors | ||
| A. H.Mansour; A. A.Thaker; Sharif Sadek; Idham A. Abed | ||
| Anbar University - College of Science | ||
| Abstract | ||
| This study was conducted for producing a single-cell protein from Whey and remnants of dates, using local yeast Isolated.The efficient Isolates were selected for utilization of the used carbon sources and production of single-cell protein.The limitation of the appropriate incubation period for growth and production as trial to improving the nutrient media components which was performed by the increment of the prepared of carbon source by focusing Whey or mixing it with the remnants of dates at different rates. The addition of supports to the media included food powder plant Northblan and remnants of culture of mushrooms and shellfish waste of bloody massacres meat and crude oil.The study also tackled the impact of the use of selected mixed culture isolates and impact of shaking and aeration processes on producing single-cell protein.The production of single cell protein was performed in shaking incubator and by using Fermenter. The most important findings are : Sexes Isolates obtained from natural sources, which included 3 Isolates of the whey environment and 3 Isolates of remnants of dates.Test results showed the efficiency of isolates selected in the production of single cell protein fermentation process for four days, the concentrated whey media gave the best production rate 4.60 g / liter. The study showed that mixing 50% of remnants of dates with 50% of concentrated whey led to significant increase in the production was 6.56 g / liter. The use of the improved materials for the media led to significant increase in the results, achieving higher productivity 8.35 g / liter when using remnants of the bloody massacres of meat. The use of mixed cultures led to significant increase in the production compared with pure cultures of Isolates that gave mixed isolates Wt2Dr1Fl higher productivity amounted to 14.79 grams / liter. The study showed that the process of agitation and aeration had significant role in raising production to hit 17.61 grams / liter when using aeration rate of 0.5 liter / minutes with shaking speed of 150 cycle / minutes.. The use of the Fermenter instead of the shaking incubator had significant increased production rate of the singlecell protein that was 23.35 grams / liter. The results of diagnostic tests showed that isolates returning to Ascomycetes, Genus of Candida, as Candida utilisWt2 and C. tropicalisDr1.The analysis of results showed that the components of single-cell protein product that the proportion of crude protein ranged between 52.55 - 57.01%, and carbohydrates 23.74 - 26.9%, and the proportion of ether extracted fat ranged 2.74 - 2.98%, the percentage of nucleic acid (3.40-3.96%). The study showed a decline in the value of BOD for the used mediato 480 mg / liter at the development mix isolates Wt2Dr1Fl . | ||
| Keywords | ||
| Candida tropicalis; Candida utilis | ||
| References | ||
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