Test the inhibition activity of two local Streptomyces isolates by used sawdust as a carbone source

Yousif Motr, Thamer

doi:10.37652/juaps.2008.15635

	Test the inhibition activity of two local Streptomyces isolates by used sawdust as a carbone source
Journal of University of Anbar for Pure Science
Article 17, Volume 2, Issue 3, 2008, Pages 69-81 PDF (630.27 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2008.15635
Author
Thamer Yousif Motr^*
Anbar University - College of Science
Abstract
Two local Streptomyces (Streptomyces O3 & Streptomyces TS36 ) was used in this study. The isolate Str. O3 has been isolated from one of Ramadi city soil, the isolate has been selected based on it's ability of producing cellulase through it's ability of digesting sawdust as culture medium to test inhibition activity against some pathogenic bacteria. The isolate Stre.TS36 prediagnosed in college of science-University of Al-Anbar has also been used. The isolate Str.O3 was described phenotypically and physiologically by many cultures media, also has been diagnosed by different temperatures, and it's ability of growth in different salt concentrations and different pH was also tested, in which selected optimum conditions to producing inhibition activity against tested pathogenic bacteria Staphylococcus aureus& E. coli.. Result showed the ability of the two selected streptomyces used in studying it's utilization of sawdust as a carbon source for growth and production of inhibition activity against pathogenic bacteria, in which the solid culture and liquid culture methods were used to grow the two local isolates to produce the inhibition activity in presence of sawdust, both isolates were able to produce the inhibition activity in both solid and liquid cultures, the isolates Str.O3 and Str.TS36 were able to produce inhibition activity in test cultures 2,3 and 4 containing 0.3, 0.7 and 1.5 g/ml of sawdust respectively against Staphylococcus aureus similar to or little better than control culture that does not contain sawdust, also the isolates Str.O3 was able of producing inhibition activity in the same previous test cultures against E. coli, the isolate Str. O3 showed high stability in high temperature in which it was able to grow and produce inhibition activity in temperature 40C0 comparing with isolate Str.TS36 that was able to grow and produce inhibition activity in 28 C0.

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