- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Bacterial Indole acetic acid ( IAA ) production by using local media and test its efficiency on soyabean plant | ||
| Journal of University of Anbar for Pure Science | ||
| Article 4, Volume 3, Issue 1, 2009, Pages 10-18 PDF (488.89 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2009.15645 | ||
| Authors | ||
| Jamal. S. Hommod* 1; Hammad F. Nawaf2; Idham A.Abed* 1 | ||
| 1Anbar University - College of Agriculture | ||
| 2Anbar University - College of Education - Al-Qaim | ||
| Abstract | ||
| This study was included isolation and identification of bacterial isolates for growth promoter production ( Indol Acetic Acid ) by application of local culture and evaluate its efficiency. According to this perpoute bacterial isolates were collected and examined for their ability to indol production.The local media used included dry powder of legume, bean ,soyabean , seed and milk powder. Its also was tested the effect of edition of supplements to the culture such as treptophan ,N. broth , glucose , N and P on indol production optimum condition was included to increase production efficincy .The ability of selection isolate for iron chelating natural, phosphate disolveing , Nitrogen fexation were tested.Applied biological expermints has been done in order to evalution the efficincy of indol production in compantion with industrial indol and also the efficincy for the selection isolates and their filteration the effect on the implantation of sow seed and groth of soyabeen which suffering from the prescence and distribution of Rhizopia espelially from lraqi soil .This study have the following results :-18 isolates were obtain cupable for them indol production ( 30 from total isolates) 14 isolats were nodulan bacteria .8 isolates which have high efficancy in indol production were tested six isolates tended to Rhizopium and two isolate to Pseudomonus in next screening used culture with special condation , two isolates Pssp2S ,Rsp8RA were selected to be used in the indol production growth on Local culture supplements The results of optimum condition study showded the best indol production acharied 41.9& 42.8 mg IAA / l respecivly This study improved the ability of selection isolates to alchelating compouned CAS from blue to pink colum with 4.5 – 5.8 min. of Ps.sp2S and Rsp8RA respectively.Their isolates have ability to phosphate dissolved in the medium with diametr range dissolving 6.25 – 11.75 mm respectively , and the ,Rsp8RA isolate has ability to nitrogen fixation in the medium with avarge of 3.65 N / L whil the Ps.sp2S doesn't able to nitrogen fixation . Result of applied biological experiment showed an increased treatment of two isolates mixture Rsp8RA , PssP2S with their filterated achaived inclues in : A-Characters of vegetative group that achaived high avarge for branching ( 4.37 ) height of plant ( 81.2 cfu/ ml and leaf area 78.4cm ) ,persente of chlorophyll ( 39.6% ), dry weight (51.2 g) Nitrogen and Phosphours yield in plant ( 491, 1020 mg ) respectively . B- Root characters were achaived high avarge the number of noduls (56.3 noduli) . weight of root group ( 10.4 gm ). c- Soil characters after sowing were achaived high average of nitrogen and phosphorus in soil (166 – 18.4 mg / kg soil) respectively and high microbial population in soil ( 7.82 Log cfu/ ml ). | ||
| References | ||
|
-الباسط ، علی سلامة وسالم، علی سمیر و الزامک فاطمة ابراهیم ولبیب ، هویدا محمد ( 2006 ). عزل وانتخاب سلالات محلیة عالیة الکفاءة من بکتریا الرایزوبیوم والازوسبیرلم والازوتوبکتر من اراضی بمحافظات الشرقیة،المجلة الزراعیة– جامعة الزقازیق مجلد 35 عدد 16: 20– 25 . 2-Rasul , G. ( 1999 ) . Production of growth hormones nitrogenase by diazotrophic bacteria and their effect on plant growth. Thesis of Ph. D. Higher Education commission. H – 9 – Islamabad.
3-Diby , P. and Sarma , Y. R. (2006). Plant growth promoting Rhizobacteria mediated root prolife ration in black pepper.
4-Jong,S.J.;Sang,S.L.;Hyoun,Y.K.;Tae,S.A.and Hong,G.S.(2003).Plant growth promotion is Soil by inoculated Micro–organisms. J. Micro.41(4):271–276.
5- Hoflich , G. ; Wiehe , W. and Buchnolz , C. H. (1995) . Rhizosphere colonization of different crops with growth promoting pseudomonas and phizobium bacteria. Micro. Res. 150 : 139 – 147.
6-Lindeman , W. C. ; Ham , G. E ( 1979) . Soybeen plant growth , nodulation and nitrogen fixation as affected by root temperatwre. Soil. Sci. Am. J. 43 : 1134 .
7- Beck , D. P. ; Materon , L. A. and Afandi , F. ( 1993 ). Practical Rhizobium legumetechnology manual. Technical manual No. 19. ICARDA.
8- Patten, C. L. , Glick B. R. 2002. Role of pseudomonas putida sindoleeacetic acid in development of the host plant root system A E M. Vol. 68. No. 8 P. 3795 – 3801 .
9- الکبیسی ، جمال صالح2008. انتاج منظم انمو اندول حامض الخلیک (IAA)اوساط محلیة واختبار کفاءته على نبات فول الصویا، اطروحة دکتوراه، کلیة الزراعة، جامعة الانبار. 10- Baily. S. M. and wiersma, N. (1975). Calculation of leaves area by equation. Plant. Ag. 54: 103 – 106.
11-Black ,C.A. 1965. Methods of soil analysis. Part(2). Chemicale and microbiological properties.Am. Soc. Arcon. Inc. publisher, Madison, Wisconsin, USA.
12- Martin , P. ; Glatzle , A ; Kolb , W. Omay, H. and Schmidt, W. (1989). N–fixing bacteria in the rhizosphere: Quantifi–cation and hormonal effect on root development. Ziet. Plant. Boden. 152(2)237–245.
13-Almonacid, S.; Quintero, N.; Martinez, and vela, M. (2000). Determination of quality parameters of bacterium inocula based on liquid for mulation elaborated with strains producing IAA. Phytopathology. 97: 462 – 468.
14-Ramazan , C. ; Mesude , F. and Erdoan , u ( 2007 ). The effect of plant growth promoting on Barley seedling Growth , nutrient uptake and some Soil properties. Turk. J. Argic : 31 : 189 – 199.
15-Fullick , W. ( 2000 ) . The effect of different concentrations of the plant growth suhstace IAA on the growth of roots and shoots. Scl – Journal. Vol. 27. No. 9 P. 212 – 217 .
16- Marcos , A. ; Brito , A. ; Sergy , G. and Hani , A. (1994). Effect of compost on Rhizosphere microflora of the tomato and on the incidence of plant growth – promoting Rhizobacteria . A E M. 61 ( 1 ) : 194 – 199 .
17-Gerte, J.; Beissner, L. and Romer, W. (2000). The quantitative effect of chemical phosphate mobilization by carboxylate aniors on uptake by single root. J. plant. Nut. and soil. Sci. 163:207–212. | ||
|
Statistics Article View: 389 PDF Download: 195 |
||