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Isolation of Protein Compounds from Elettaria cardamomum Fruits Plant and | ||
| Rafidain Journal of Science | ||
| Article 19, Volume 27, Issue 4, 2018, Pages 53-63 PDF (546.45 K) | ||
| DOI: 10.33899/rjs.2018.159346 | ||
| Authors | ||
| Nahida S. Al-Chalabi* 1; Rasha H. Al –Juboory2 | ||
| 1Department of Chemistry, College of Education for Pure Science,University of Mosul | ||
| 2Department of Chemistry, College of Education for Pure Science, University of Mosul | ||
| Abstract | ||
| This study included preparation of aqueous extract for fruits of Elettaria cardamomum plant, isolation of their proteins and precipitation takes place by using acetone. The precipitate of the isolated proteins were purified using gel filtration chromatography depending on the volume of their molecules (proteins) and their molecular weights by a gel of Sephadex G – 75. The results were shown that there are two protein compounds A and B isolated from the protein precipitate resulted from cold aqueous extract for this plant by gel filtration chromatography. Then the molecular weight was determined for these compounds isolated using the same techniques (gel filtration) by comparison these compounds with proteins known molecular weights. The approximate molecular weight of two compounds A and B are 29940, 10627 Dalton respectively | ||
| Keywords | ||
| Elettaria cardamomum; protein; Gel filtration; aqueous extract | ||
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