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Regeneration of White Lupin, Lupinus albus, Plants form Hypocotyl Stems | ||
| Rafidain Journal of Science | ||
| Article 23, Volume 27, Issue 4, 2018, Pages 95-102 PDF (613.48 K) | ||
| DOI: 10.33899/rjs.2018.159348 | ||
| Authors | ||
| Amjad A. Mohammed* 1; Rana T. Yahya2 | ||
| 1Department of Biology , College of Science, University of Mosul | ||
| 2Department of Biophysics, College of Science, University of Mosul | ||
| Abstract | ||
| This study succeeded in regenerating the white lupin, Lupinus albus, plants from differentiated the hypocotyl stems callus of its sterilized seedlings. The results indicated to superior the solid Murashige and Skoog (MS) medium provided with 1.0 mg L-1 NAA in callus initiation with 90% after 20 days of culture from the other of selected media. The rate of the value of the callus fresh weight was 3.3 gm after 40 days of growth, which gives indication on the viability and activity of callus. The continued of callus subculture on the same its initiated medium led to spontaneous production of 22 shoots. These regenerated shoots rooted when cultivated in MSO with its full strength at ratio 50% after 26 days, producing the whole lupin plants in the culture media. . | ||
| Keywords | ||
| White lupin plants; callus differentiation; fresh weight | ||
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