- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Detection of Ochratoxigenic Potential in some Aspergillus and Penicillium Isolates from Vineyard Soil, Fresh and Dried Grapes by ELISA | ||
| Rafidain Journal of Science | ||
| Article 1, Volume 27, Issue 4, 2018, Pages 1-7 PDF (238.37 K) | ||
| DOI: 10.33899/rjs.2018.159364 | ||
| Authors | ||
| Asia Saadullah* 1; Samir K. Abdullah2 | ||
| 1Department of Biology, College of Science, University of Duhok | ||
| 2Department of Biology, College of Science, University of Zakho | ||
| Abstract | ||
| Several species of Aspergillus and Penicillium were isolated and identified from samples of vineyard soil, fresh grape berries and dried vine fruits collected from grapevine nursery and shops in Duhok province. The ochratoxigenic potential of some of their strains was evaluated by ELISA technique. Ochratoxin A (OA) was detected in cultures of two species of section Nigri (A.carbonarius and A.niger aggreg.), two species of Aspergillus section Circumdati (A.ochraceus and A.westerdijkia) and one species of OA was found at levels from 0.64 to 0.72 ng ml in A.carbonarius isolates, from 0.64 to 0.66 ng ml in A.niger aggreg. isolates, from 0.23 to 0.65 ng ml in A.ochraceus isolates and 0.40 ngml in A.westterdijkia isolate, whereas the level was from 0.61 to 0.64 ngml in Penicillium verrucosum.تم عزل وتشخیص العدید من الأنواع الفطریة العائدة للجنسین اسبرجلس و بنیسیلیوم من عینات ترب حقول الأعناب فضلا عن ثمار العنب الطریة و الجافة والتی جمعت من محافظه دهوک. تم الکشف عن قابلیه إنتاج هذه العزلات لإنتاج السم Aspergillus section Nigri تم الکشف عن إنتاج السم من قبل عزلتان من ELISA. الفطری اوکراتوکسین باستخدام تقنیه Aspergillus section Circumdati (A.ochraceus وعزلتان من (A.carbonarius A.niger aggreg.), .Penicillium (P.verrucosum و عزله من A.westerdijkiae A.niger 0.64 نانوغرام / ملغم وفى عزلات - ما بین 0.72 A.carbonarius تراوح مستوى إنتاج السم من قبل عزلات 0.23 و فی عزله - مابین 0.65 A.ochraceus 0.64 نانوغرام/ ملغرام وفى عزلات - مابین 0.66 aggreg. 0.61 نانوغرام/ ملغم فى عزلات - 0.40 نانوغرام/ ملغرام بینما تراوح الإنتاج مابین - 0.64 A.westerdijkiaie .Penicillium verrucosum | ||
| Keywords | ||
| Ochratoxin A; aspergillus; Penicillium; grapevine; ELISA | ||
|
Statistics Article View: 541 PDF Download: 347 |
||