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Isolation of Acetylcholinesterase Enzyme from Cerebrospinal Fluid of Healthy and Hydrocephalus Disease Patients’ Children in Mosul City | ||
| Rafidain Journal of Science | ||
| Article 47, Volume 27, Issue 4, 2018, Pages 374-387 PDF (921.17 K) | ||
| DOI: 10.33899/rjs.2018.159372 | ||
| Authors | ||
| Lelas F. Bdaiwi; Luay A. Al-Helaly; Sayran S. Saleh | ||
| Abstract | ||
| The research includes the partial isolation of acetylcholinesterase enzyme, from CSF of hydrocephalus patients and healthy children, the results showed that there was one peak with high activity from gel filtration chromatography using sephadex G-100 for protienous solution resulted from ammonium sulfate precipitation (70%) after dialysis process, with purification folds up to (27,17) times of patients and healthy children respectively.Another column was used with sephadex G-200 and the results showed two protienous peak for Acetylcholinesterase with purification folds up to (33, 24) times of patients and healthy children respectively, the second protienous peak had higher activity than the first peak. The results showed also that the enzyme which was isolated from CSF of patients children had a higher activity than the enzyme isolated from CSF of healthy children, the peak with high activity was used to determine the comparative molecular weight using gel filtration chromatography with sephadex G-200.which was (163606 ± 4000) Dalton. G-200. | ||
| Keywords | ||
| Hydrocephalus; Cerebrospinal fluid; Acetylcholinesterase Enzyme | ||
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