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Qualitative and Quantitative Determination of Salicylic acid and Resorcinol in Callus of Calendula officinalis L. Using HPLC Technique | ||
| Rafidain Journal of Science | ||
| Article 8, Volume 27, Issue 3, 2018, Pages 1-14 PDF (1.17 M) | ||
| DOI: 10.33899/rjs.2018.159375 | ||
| Authors | ||
| Bashar Z. Kassab Bashi* ; Khawla M. Al- Naoh | ||
| Horticulture Science and Landscape Design, College of Agriculture and Forestry, University of Mosul | ||
| Abstract | ||
| Parts of leave of Calendula officinalis plant before and after blooming were cultured on MS medium supplemented with 30 g / l scores, 6 g / l agar, pH was 5.7± 0.1, in addition to 2,4-D at (0.0 , 0.1 , 0.2 , 0.5) mg/ l for callus induction, produced callus recultured on medium supplemented with ( 0.0, 0.5, 1.0, 1.5, 2.0) mg/ l NAA interaction with 0.1 mg/ l BA for three times three weeks for each one. Salicylic acid and Resorcinol were stimulated and identified in callus using High Performance Liquid Chromatography (HPLC) method. The data indicate: highest fresh weight of callus 13.04 gm achieved from cultured parts of leaves before flowering on medium supplemented with 1.0 mg/ l NAA and 0.1 mg/ l BA after nine weeks, and the highest fresh weight of callus 20.50 g obtained from cultured parts of leaves during flowering on medium supplemented with 1.5 mg/ l NAA and 0.1 mg/ l BA after nine weeks, highest quantity of salicylic acid 30.02 mg/gm dry weight was obtained from callus produced from parts of leaves before flowering cultured on MS medium supplemented with 1.5 mg/ l NAA and 0.1 mg/ l BA after six weeks, highest quantity of Resorcinol 139.28 mg/ gm dry weight was obtained from callus produced from parts of leaves took during flowering cultured on MS medium supplemented with 2.0 mg/l NAA and 0.1mg/ l BA after six weeks. | ||
| Keywords | ||
| Calendula officinalis L; Salicylic acid; Resorcinol; callus | ||
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