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Identification of Vinblastine and Vincristine in Callus of Catharanthus roseus L. by High Performance Liquid Chromatography (HPLC) Apparatus | ||
| Rafidain Journal of Science | ||
| Article 10, Volume 27, Issue 3, 2018, Pages 23-34 PDF (1.19 M) | ||
| DOI: 10.33899/rjs.2018.159376 | ||
| Authors | ||
| Aysar M. Almemary* 1; Bashar Z. Kassab Bashi1; Ayad C. Aldaoody2 | ||
| 1Department of Horticulture Science and Landscape Design, College of Agriculture and Forestry, University of Mosul | ||
| 2Department of Biology, College of Education for Pure Science, University of Mosul | ||
| Abstract | ||
| Parts of leaves, nodal explant, and internodes of Catharanthus roseus were cultured on solid MS medium supplemented with 30 gm / L sucrose, 6 mg / L agar with addition BA at 0.0, 0.1, 0.2, 0.3, 0.5 mg/ L with 0.75 mg /L 2,4-D for callus initiation and growth. Vinblastine and Vincristine were identified in these calli by using High Performance Liquid Chromatography.(HPLC). Data refers: highest fresh weight of callus and highest protein percentage 1.907 gm, 14.47% were achieved respectively from cultured parts of leaves on MS medium supplemented with 0.3 mg/ L BA with 0.75 mg/L 2,4-D. cultured nodal explant on MS medium supplemented with 0.75 mg/ L 2, 4-D produced highest fresh weight of callus and highest protein percentage 0.496 gm, 9.15% respectively, culture internodes explant on MS medium supplemented with 0.2 mg/ L BA and 0.75 mg/ L 2,4-D produced highest fresh weight of callus and highest protein percentage 0.717 gm, 11.90 % respectively. The callus produced from cultured parts of leave on MS medium supplemented with 0.3 mg/ L BA and 0.75 mg/L 2,4-D gave highest absorption value for Vinblastine at retention time (13.536) with detected area (255647). highest detected area was (975021) at retention time (18.886) for Vincristine assessed from callus produced from cultured parts of leave on MS medium supplemented with 0.75 mg/L 2,4-D, callus produced from cultured nodes on MS medium supplemented with 0.2 mg/ L BA and 0.75 mg/ L 2,4-D gave highest detected area for Vinblastine (318395) at retention time (12.809) but culture nodes on MS medium supplemented with 0.3 mg/ L BA with 0.75 mg/L 2,4-D gave highest detected area (577979) for Vincristine at retention time (18.139), callus produced from cultured internodes on MS medium supplemented with 0.2 mg/ L BA and 0.75 mg/ L 2,4-D gave highest absorption value for Vinblastine and Vincristine (1892650) and (964402) at retention time (13.632) (18.309) respectively.. | ||
| Keywords | ||
| Catharanthus roseus plants; callus; Vinblastine; Vincristine | ||
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