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First phylogenetic characterization of Pseudocowpox virus from cattle in Al-Qadisiyah province/ Iraq | ||
| Iraqi Journal of Veterinary Sciences | ||
| Article 20, Volume 33, Issue 1, 2019, Pages 123-126 PDF (1.02 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/ijvs.2019.125525.1047 | ||
| Authors | ||
| Salah Mahdi Karim1; Khalefa Ali Mansour1; Ali Hassan Janabi* 2; Nawras K. M. Al-Nakeeb2 | ||
| 1Department of veterinary internal and preventive medicine, College of veterinary medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq | ||
| 2Department of Microbiology, College of veterinary medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq | ||
| Abstract | ||
| This study was initiated for the first time for identification, using sequencing and phylogenetic analyses, of pseudocowpox PCPV that inhabit dairy cows in Al-Qadisiyah province, Iraq. Scab sampling was performed to obtain specimens from udder and teats of 18 affected cows. Initially, a polymerase chain reaction (PCR) method was followed to target a 408-bp piece of the GM_CSF/IL-2 inhibition factor gene (GIF) that belongs to PCPV. Then, the PCR products were sent out to partial sequencing of the GIF gene. The results of the PCR have indicated the presence of the virus in only 3 out of 18 samples. When the sequences were studied using phylogeny, the results have revealed that one of our PCPV strains has a close matching with some of the world strains such as from New Zealand. While two of the current study strains have clustered together with a strain from Finland. The results of our study confirm the presence of the PCPV in dairy cows that induces milker’s nodules. | ||
| Keywords | ||
| Dairy cows; <i>GIF</i> gene; PCR; Pseudocowpox virus; Sequencing | ||
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