Khaleel, T. (2018). Characterization of celery leaves peroxidase. , 5(5), 108-124.
Tareq Nawaf Khaleel. "Characterization of celery leaves peroxidase". , 5, 5, 2018, 108-124.
Khaleel, T. (2018). 'Characterization of celery leaves peroxidase', , 5(5), pp. 108-124.
Khaleel, T. Characterization of celery leaves peroxidase. , 2018; 5(5): 108-124.

Characterization of celery leaves peroxidase

Journal of Kerbala for Agricultural Sciences
Article 17, Volume 5, Issue 5, 2018, Pages 108-124    PDF (879.96 K)
Author
Tareq Nawaf Khaleel*
Food since dep. / college of agriculture and forestry / university of Mosul
Abstract
This research was conducted in food sciences laboratories, college of agricultural and forestry university of  mosul and end in 2017 was aimed to isolate and characterization peroxidase enzyme from celery leaves by water extraction followed by precipitation using different concentrations of acetone ( 1:1 , 1.5:1 , 2:1 acetone : filtrate ). The best ratio was found to be 1.5:1 which gave 74,3%.
Some of the enzyme properties were studied. Optimum temperature for enzyme activity was 40C. The enzyme was found to be relatively thermostable. It  retained all initial activity after 60 minutes of incubation at 50C and 77% at 60C . However, the enzyme had lost all activity upon incubation at 80C for 60 minutes. Optimum pH of activity was 6.3 . Optimal stability was found at pH 5.5 using citrate buffer. Activation energy for the enzyme activity was 3,261k cal.mol-1, and for enzyme denaturation was 6,131k cal.mol-1. Michaelis constant (Km) and maximum velocity (Vmax) were calculated to be 20 mM and 2 µmol. Min-1, respectively using guaiacol as substrate.
Keywords
peroxidase; celery leaves; Characterization
References
  1. Agostini, E.; Hernandez-Ruiz, J.; Arnao, M. B.; Milrand, S. R.; Tigier, H. A. and Acosta, M. (2002) A peroxidase isoenzyme secreted by turnip (Brassica napus) hairy-root culture inactivation by hydrogen peroxide and application in diagnostic kit,” Biotechnology and Applied Biochemistry, vol. 35, no. 1, pp. 1-7.
  2. Al-badri R. H. (2012)  Extraction, Partial Purification and Characterization of Peroxidase from Malva neglecta, Academic Press college of sciences universty of alnahreen October.
  3. Ali, L. F. (2008) Isolation and study of peroxidase properties from local Cabbage (Brassica,Oleracea Var. capitate L.).
  4. Almagro, L.; Gomez Ros, LV.; Belchi-Navarro, S. B. R.; Ros Barcelo, A. and Pedreno, M. (2009) Class III peroxidases in plant defence reactions. Journal of Experimental Botany 60: 377 – 390.
  5. Bakalovic, N.; Passardi, F.; Ioannidis, V.; Cosio, C.; Penel, C.; Falquet, L. and Dunand, C. (2006) A class III plant peroxidase database. Phytochemistry 67: 534–539.
  6. Belcarz, A.; Ginalska, G.; Kowalewska, B. and Kulesza, P. (2008) Spring cabbage peroxidase - potential tool in biocatalysis and bioelectrocatalysis,” Phytochemistry, vol. 69, no. 3, pp. 627-636.
  7. Bender, J. P.; Mazutti M. A.; Di Luccio M. And Treichel H. (2008) Extraction of Inulinase Obtained by Solid State Fermentation of Sugarcane Bagasse by Kluyveromyces marxianus NRRL Y-7571, Appl. Biochem. Biotechnol, 149: 195–203.
  8. Berlina, A. N.; Zherdev, A. V.; Dzantiev, B. B.; and Sakharov, I. Y. (2007) Use of soybean peroxidase for the enzyme immunoassay of sulfamethoxipyridazine in milk,” Applied Biochem Microbiol., vol.43, no. 5, pp. 550-555.
  9. Cai, C.; Xu, C.; Li, X.; Ferguson, I. and Chen, K. (2006) Accu­mulation of lignin in relation to change in activities of lignification enzymes in loquat fruit flesh after harvest. Postharvest Biology and Technology, 40: 163–169.
  10. Carvalho, A. S. L.; Melo, E. P. E.; Ferreira, B. S.; Neves-Petersen, M. T.; Peterson, S. T. and Aires-Barros, M. R. (2003) Heme and pH-dependent stability of an anionic horseradish peroxidase,” Biochemistry and Biophysics, vol. 415, no. 2, pp. 257-267.
  11. Copeland, R. A. (1996) Enzymes: A Practical introduction to structure mechanism and data analysis. P: 167, 175-178, Wiley-Vch, Inc. New York.
  12. Cosio, C. and Dunand, C. (2008) Specific functions of individual class III peroxidase genes. J. Exp. Bot. 60:391-408.
  13. Dalaly, B. K. and Rikaby, K. (1988) Food chemistry second edition, directors of books homeuniversity of Mosul.
  14. De Gara, L. (2004) Class III peroxidases and ascorbate metabolism in plants Phytochemistry Reviews .3(1-2): 195-205.
  15. Fagerstedt, K.V.; Kukkola, E.M.; Koistinen, V.T.; Takahashi, J. and Marjamaa, K. (2010) Cell WallLignin is Polymerised by Class III Secretable Plant Peroxidases in Norwa Spruce .J. Integative Plant Biology.52(2):186-194.
  16. Fatima, A.; Husain, Q. and Khan, R. H. (2007) A peroxidase from bitter gourd (momordica charantia) with enhanced stability against organic solvent and detergent: A comparison with horseradish peroxidase,” Journal of Molecular Catalysis B: Enzymatic, vol.47, no. 1, pp. 66-71.
  17. Gill, P. K.; Manhas, R. K. and Singh, P. (2005) Bioresource Technology, 97, 894–902.
  18. Gonzalez-Barrio, R.; Salmenkallio-Marttila, M.; Tomas-Bar­beran, F.A.; Cantos, E.and Espin J.C. (2005) Etiology of UV-C-induced browning in var. Superior white table grapes. Journal of Agricultural and Food Chemistry, 53: 5990–5996.
  19. Gutierrez, J.; Lopez N.M.J.; Gomez-Ros L.V.; Novo U. E.; Esteban-Carrasco, A.; Diaz, J.; Sottomayor, M.; Cuello, J. and Ros-Barcelo, A. (2009) Hormonal regulation of the basic peroxidase isoenzymefrom Zinnia elegans. Planta 230:767-778.
  20. Hamid, M. and Rehman, K. U. (2009) Potential applications of peroxidase, Food Chemistry, vol. 115, no. 1, pp. 1177-1186.
  21. Holschuh, H. J.; and  de Doutorado T. (2000) Universidade Estadual de Campinas, Brasil.
  22. Husain, M. and Husain, Q. (2008) Applications of redox mediators in the treatment of organic pollutants by using oxidoreductive enzymes A review Crit. Rev. Environ. Sci.Technol.38:1–42.
  23. Joy, O. and Eze, S. O.O. (2015) Partial Purification and Characterizaton of Peroxidase Extracted from Gongronema latifolium American-Eurasian Journal of Scientific Research 10 (4): 221-227.
  24. Kalsoom, U.; Bhatti, H. N.; Bhatti I. A. and Asgher M. (2010) Kinetics and thermodynamics of thermal inactivation of peroxidase from cauliflower, Asian J. Chem. 22: 7883-7890.
  25. Kharatmol, P. P. and Pandit, A. B. (2012) Extraction Partil purfication and charactrization of acidic peroxidase from cabbage leaves.
  26. Kumar, P.; Kamle, M.; Singh, J. and Rao, D.P. (2008) Isolation and characterization of peroxidase from the leaves of Ricinus communis .J. Biotechnology and Biochemistry.
  27. Kuzaniak, E. and Sklodowska, M. (2005) Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants. Planta 222:192-200.
  28. Lalida P. S.; Kijjanapanich, P. And Phutrakul, S. (2015) Characterization of Partially Purified Peroxidase from Fingerroot (Boesenbergia Rotunda (L.) Mansf.)  Journal  of Medical and Bioengineering Vol. 4, No. 3.
  29. Liu, W.; Kumar, J.; Tripathy, S.; Senecal, K. J. and Samuelson, L. (1999) Enzymatically synthesized conducting polyaniline,” Journal of American Chemical Society, vol. 121, no.1, pp. 71-78.
  30. Maciel, H. P.; Gouvea, C. M.; Toyama, M.; Smolka, M.; Marangoni, S. and Pastore, G. M. (2007) extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves, Quim. Nova, Vol. 30, No. 5, 1067-1071.
  31. Mamounata, D.; Oumou, H. K.; Nafissetou, O.; Romaric, G. B.; Imael, H. N. B. and Mamoudou, H. D. (2011) Comparison of peroxidase activity from Allium sativum, Ipomoea batatas,Raphanus sativus and Sorghum bicolor grown in Burkina Faso. African  Journal of Biochemistry Research, 5 124-128.
  32. Mika, A.; Minibayeva, F.; Beckett, R. and Lüthje, S. (2004) Possible functions of extracellular peroxidases in stress-induced generation and detoxification of active oxygen species. Phytochemistry .Reviews .3(1-2):173-193.
  33. Mizobutsi, G. P.; Finger, F. L.; Ribeiro, R. A.; Puschmann, R.; Neves, L. L. and Mota, W. F. (2010) Effect of pH and temperature on peroxidase and polyphenoloxidase activities of litchi pericarp Sci. Agric. (Piracicaba, Braz.), v.67, n.2, p.213-217.
  34. Nadaroglu, H.; Celebi, N.; Demir, N. and Demir, Y. (2013) Purification and characterisation of a plant peroxidase from rocket (Eruca vesicaria sbsp. Sativa) (Mill.) (syn. E. sativa) and effects of some chemicals on peroxidase activity in vitro. Vol. 8(21), pp. 2520-2528.
  35. Nouren, S.; Bhatti, H. N.; Bhatti I. A. and Asgher M. (2013) Kinetic and thermal characterazation of peroxidase from peels of citrus reticulata var kinnow The Journal of Animal & Plant Sciences, 23(2): Page: 430-435 ISSN: 1018-7081.
  36. Padigila, A.; Medda, R. and Cruciani, E. (1995) Fractionation and Characterization of two form of peroxidase from Oryza sativa. Preparative Biochemistry. 25(1&2): 11-19.
  37. Pinto, M. S. T.; Ribeiro, J. M.; de Araújo, F. P.; de Melo, N. F. and Fernandes, K. V. S. (2015) Purification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.) Vol. 14(21), pp. 1838 – 1845.
  38. Regalado, C.; García-Almendárez, B. E. and Duarte-Vázquez, M. A. (2004) Biotechnology applications of  peroxidases, Phytochemistry Reviews, vol.3, no.1, pp.243-256.
  39. Robinson, D.S.; Eskin, N.A.M. (eds) (1991) Oxidative En­zymes in Foods. New York, Elsevier: 217–273.
  40. Ros-Barceló, A. and Pomar, F. (2002) Plant peroxidases: versatile catalysts in the synthesis of bioactive natural products. Stud. Nat. Prod. Chem. 27:735-792.
  41. Saeidian, S. and Ghasemifar, E. (2013) Effect of temperature on guaiacol Peroxidase of Pyrus communis ISSN: 2300-9675, Vol. 5, pp 46-51.
  42. Segel, I. H. (1976) Biochemical calculation, 2nd edition, John Wiley and Sons. New York.
  43. Shahriar, S. and Elham, G. (2013) Effect of temperature on guaiacol Peroxidase of Pyrus communis. Int. Lett. Nat. Sci. 5: 46-51.
  44. Silva and Valdir (1990) Soluble and Bound peroxidase from papaya fruit. Phytochemistry. 29(4): 1051-1056.
  45. Singh, J.; Dubey, A.; Diwakar, S. K.; Rawat, S. K.; Batra, N. and Joshi, A. (2010) Biochemical Characterization of Peroxidases from the Fruits of Mallus pumilus (ISSN: 2141-5153) Vol. 1(4) pp.050-058.
  46. Suha, O.A.; Babiker, E.M. and Babiker, E.E. (2013) Thermostability at different pH levels of peroxidase extracted from four vegetables. Int. Food Res. J. 20(2):715-719.
  47. Sukalovic, V.H.T.; Veljovic-Jovanović S.; Dragisic Maksimovic, J.; Maksimovic, V. and Pajić, Z. (2010) Characterisation of phenol oxidase and peroxidase from maize silk.Plant Biology. 12(3):406-413.
  48. Thongsook, T. and Barrett, D. (2005) Purification and partial characterization of broccoli (Brassica Var. Italica) peroxidase. J. Agric. Food Chem. 53(8):3206-14.
  49. Ward, G.; Hardar, Y. and Dosoretz, C. G. (2001) Inactivation of lignin peroxidase during oxidation of the highly reactive substrate ferulic acid,” Enzyme Microbiology Technology, vol. 29, no. 1, pp. 34-41.
  50. Whitaker, J. R. (1972) Principle of enzymology for food science. Marcel Dekker, Inc., New York.
  51. Yazdi, M. T.; Khaleghparast, S. and Nayebpour, S. M. (1998) Purification and partial characterization of Peroxidase from Cultivated Raphanus Sativus L. var. cicil, mjiri, Vol. 12, No.3, 273-277.
  52. Yihong, H.; Juan, W.; Ping L. and Yiwei, M. (2012) Purification and partial characterization of peroxidase from lettuce stems. African Journal of Biotechnology, 11: 2752-2756.
  53. Zhang, X. and Shao, X. (2015) Characterisation of Polyphenol Oxidase and Peroxidase and the Role in Browning of Loquat Fruit Czech J. Food Sci., 33, (2): 109–117.
Statistics
Article View: 214
PDF Download: 504


Journal Management System. Powered by iJournalPro.com