Detection of the Genetic Stability for the Tissue Digitalis lanata Plants that Seeds Irradiation with Gamma Ray by Using RAPD Technique

Almukhtar, Sarab A.; Kahdim, Zaid K.; Mahdi, Aymen J.

	Detection of the Genetic Stability for the Tissue Digitalis lanata Plants that Seeds Irradiation with Gamma Ray by Using RAPD Technique
Journal of Kerbala for Agricultural Sciences
Article 1, Volume 5, Issue 1, 2018, Pages 65-80 PDF (1.02 M)
Authors
Sarab A. Almukhtar^* ¹; Zaid K. Kahdim¹; Aymen J. Mahdi²
¹College of Agriculture – Kerbala University
²College of pharmacy – Kerbala University
Abstract
Randomized Amplified Polymorphic DNA (RAPD) technique was used to validate the genetic stability of the digitalis plantlets produced by irradiated seeds by (0, 10, 20, 30) doses of gamma irradiation to stimulate their germination and growth. The irradiated seed was planted on the growth regulator-free MS medium. DNA print tests were performed by using RAPD markers. DNA was extracted from the green leaf samples of the tissue culture plants for each treatment with control at 1 month age. After determining the optimal conditions for RAPD reactions, usingeight starters OPA11, OPA7, OPA7, OPA7, OPC2, OPA11, OPA2 and OP511, with a perfect match in the distribution pattern of the packages for the irradiated treatments. RAPD markers proved to be an easy and rapid DNA marker for genetic compatibility. Therefore, the study proved that the doses used are catalysts and this is the purpose of their use, and there was no genetic heterogeneity in the irradiated seeds or the resulting plants compared with the treatment of non-irradiated control.
Keywords
Digitalis lanata; genetic stability; RAPD technique; Gamma Ray; In Vitro

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