The inhibitory effect of the pomegranate extract (Punica granatum) towards certain species of species Lactobacillus and Clostridium isolated from human faeces

Hekmat, Ahmed Talal; Al - Obeidi, Omar Muayed

doi:10.33899/edusj.2019.161049

	The inhibitory effect of the pomegranate extract (Punica granatum) towards certain species of species Lactobacillus and Clostridium isolated from human faeces
Journal of Education and Science
Article 15, Volume 28, Issue 1, 2019, Pages 172-186 PDF (721.2 K)
Document Type: Research Paper
DOI: 10.33899/edusj.2019.161049
Authors
Ahmed Talal Hekmat; Omar Muayed Al - Obeidi
Abstract
This study showed isolation and diagnosis of 70 isolates of intestinal flora that isolated from feces of healthy people in Mosul city / Iraq, distributed to 40 isolates from beneficial bacteria Lactobacillus spp. and 30 isolates from harmful bacteria Clostridium spp., this include species of beneficial bacteria : L. acidophilus, L. casei subsp. casei, L. pentosus, L. reuteri, L. ruminis, L. fermentum, L. gasseri, L. helveticus, L. plantarum, L. paracasei subsp. paracasei, L. salivarius, L. casei subsp. rhamnosus and L. johnsonii, as well as the species of harmful bacteria included into C. butyricum, C. difficile, C. cadaveris, C. septicum, C. ramosum, C. histolyticum, C. tertium, C. perfringes and C. clostridiiforme, adopted cultural qualities, microscopic and biochemical tests to get the results. Inhibitory effectiveness of the variable constrations 4, 8 and 12 mg. from alcoholic (methanol) extract of peeled fruits of pomegranate (Punica granatum) against the isolated bacteria was studied. The method of disks in the measurement of the effectiveness was adopted. Results showed that the plant extract has a powerful effect against pathogenic bacteria represented by Clostridium spp. while lower activity against bacteria Lactobacillus spp.

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