EFFECTS OF STATIC MAGNETIC FIELD ON Escherichia coli MUTATION

Kamel, Fouad; Amin, Ashti M.; Qader, Saleem Saaed

doi:10.33899/magrj.2018.161390

	EFFECTS OF STATIC MAGNETIC FIELD ON Escherichia coli MUTATION
Mesopotamia Journal of Agriculture
Article 28, Volume 46, Issue 1, 2018, Pages 265-270 PDF (312.96 K)
Document Type: Research
DOI: 10.33899/magrj.2018.161390
Authors
Fouad Kamel^* ¹; Ashti M. Amin²; Saleem Saaed Qader²
¹Medical Technical Institute, Erbil. Polytechnic University, Erbil, Iraq
²Hawler Medical Research Centre, Erbil University, Iraq.
Abstract
The effects of a dipolar static magnetic field of strength 400, 800, 1200 and 1600 Gauss were prepared locally, on the ultra-structure of E. coli type 1 bacterium cells have been studied. Equal volumes of liquid culture media were exposed to the magnetic field for different periods, the three most effective periods, namely: 24 hrs, 48 hrs and 72 hrs were chosen for all our experimental studies. After API kit test of treated E. coli culture media as control group. Results indicated that exposure of the microorganisms to the demonstrated magnetic field caused pronounced changes in the Arginine dihydrolase (ADH), Citrate utilization (CIT) and Gelatinase (GEL) were observed on the cell growth. Besides, changes in the morphology of the E. coli colonies were observed after exposure period and subculture on MacConkey agar. Furthermore, the bacterial growth subculture tested for morphological and biological activity, the results suggested that a mutation occurred in bacterial cells.
Keywords
magnetic field; Optical density; E coli; mutation

Full Text
درست تأثیر المجال المغناطیسی ثنائی القطب وبقوى 400،800،1200،1600 کاوس المحضرة محلیا على الترکیب البنائی لبکتریا type 1.E.coli استعملت احجام متساویة من مرق الوسط الزرعی السائل للبکتریا وعرضت للمجالات المغناطیسیة المختلفة ولفترات زمنیة متفاوتة والمتضمن 24, 48 و 72 ساعة کل على انفراد مقارنة بنموذج السیطرة السالبة للمزروع البکتیری غیر المعرضة للمجال المغناطیسی. اختبر مزروع البکتریا المعویة المعاملة باختبار API kit, اشارة النتائج حدوث تغییر فی نتائج الاختبارات (Arginine dihydrolase (ADH), Citrate utilization (CIT Gelatinase (GEL). کما لوحظ تغییر فی شکل مستعمرات البکتریا المعاملة على الوسط MacConkey agar. استنتج من نتائج دراسة شکل المستعمرات وفعالیتها الحیاتیة حدوث الطفرة لخلایا البکتریا المعرضة للمجال المغناطیسی.
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