Effect of Agricultural Exploitation on the Activity of Alkaline Phosphatase and Its Kinetic Properties in Some Soils

Salih Jabbar Al-Taweel, Luma; Abedulkadhim Alwan Al- Jubouri, Gaith

doi:10.33794/qjas.2019.162668

	Effect of Agricultural Exploitation on the Activity of Alkaline Phosphatase and Its Kinetic Properties in Some Soils
Al-Qadisiyah Journal For Agriculture Sciences
Article 1, Volume 9, Issue 1, 2019, Pages 72-87 PDF (0 K)
DOI: 10.33794/qjas.2019.162668
Authors
Luma Salih Jabbar Al-Taweel; Gaith Abedulkadhim Alwan Al- Jubouri
College of Agriculture / University of Al-Qadisiyah, Iraq
Abstract
In order to study the role of agricultural exploitation in the activity of Alkaline phosphatase in the province of Diwaniyah, six sites that differ in some of their chemical, physical and biological properties were selected (Diwaniyah, Sunniya, Shamiya, Daghara, Afak and Al Budair). Three types of soils were chosen in each location, namely, orchard soil, field soil and jungle soil. The kinetic parameters of the enzymes maximum velocity (Vmax) and Michaelis constant (Km) were estimated in all study soils using increasing concentrations of the controlled substance. The results present that the highest efficacy of this enzyme is in the orchard soil of all sites except the location of the center Diwaniyah and Shamia. The average efficacy values ranged between (208.11 - 234.95) μg P-Nitro phenol. g-1 soil. 1 hour-1. The highest value of maximum velocity (Vmax) recorded at the field soil of Al-Shamia site (108.57) μg P-Nitro phenol. g-1 soil. 1 hour-1. The lowest value recorded at the soil of a jungle field at the Sunniya location (49.62) μg P-nitro phenol. g-1 soil. 1 hour-1. While the orchard soil of Al-Budair had the highest value of the Michaelis constant (Km) of (85.90) mM, the orchard soil of the Sunniya location had the lowest value (24.34) mM. .
Keywords
agricultural exploitation; Asparaginase; Vmax; Michaelis constant

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