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Detection of Extended Spectrum β-lactamases Producing by Klebsiella pneumonia Isolated from Urinary tract Infection Patients by Using mPCR Technique | ||
| Al-Qadisiah Medical Journal | ||
| Article 1, Volume 12, Issue 2, 2016, Pages 59-64 PDF (0 K) | ||
| Author | ||
| Wafaa Abdelwahed Jaheel Al-Kaaby | ||
| Abstract | ||
| Objective: In this study Klebsiella pneumonia where isolated from urine samples and detection of extended spectrum β-Lactamases by using mPCR technique. Method: This isolated subjected to the antimicrobial susceptibility test (Disc diffusion) for some antibiotics following by multiplex PCR techniques for detection extended spectrum β-Lactamase genes (bla TEM, bla SHV, bla CTX-M and bla AMPC). Results: The extended spectrum β-Lactamase genes by PCR techniques were given bla CTX-M (30/93.75%), bla SHV (25/78.12%), bla TEM (18/56.25%) and bla AMPC (22/68.15%), respectively. Conclusion: K. pneumonia isolates of urinary tract infection patients highly associated with the emergence of bla CTX-M β-Lactamase that provides useful good treatment. | ||
| Keywords | ||
| pneumonia; Lactamase; antimicrobial | ||
|
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