Spectrophotometric determination of metoclopramide hydrochloride in bulk and in pharmaceutical preparations

A. Al-Hamody, Intesar; N. Al-Sabha, A; Theia,

	Spectrophotometric determination of metoclopramide hydrochloride in bulk and in pharmaceutical preparations
Iraqi National Journal Of Chemistry
Article 1, Volume 0, Issue 24, 2014, Pages 561-570
Authors
Intesar A. Al-Hamody; a N. Al-Sabha;
Abstract
Abstract A rapid, simple, reproducible and sensitive spectrophotometric method for assay of metoclopramide hydrochloride was investigated. The method is based on the interaction of metoclopramide hydrochloride with 2,4-dinitro-1-flourobenzene reagent to give a highly coloured species with maximum absorption at 315 nm in aqueous solution in the presence of sodium hydroxide. Beer's law was obeyed in the range of 1.0-28 g/ml with a molar absorptivity of 8.007×103 l.mol-1.cm-1 and the limit of detection was 0.01858 g/ml. The accuracy (average recovery %) was 101.45 % and the relative standard deviation (RSD) was better than 1.3%. Also, it was found that the product formed was in ratio of 1:1 species with stability constant of 2.975106 L/mol. The method was applied successfully to the assay of metoclopramide hydrochloride in pharmaceutical formulations and was agreed well with its certified value and with those of standard addition and British pharmacopoeia methods.
Keywords
Spectrophotometry; dinitro; fluorobenzene; metoclopramide

Statistics Article View: 193 PDF Download: 27