- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Study of histopathological and biochemical effect of Punica granatum L. extract on streptozotocin -induced diabetes in rabbits | ||
| Iraqi Journal of Veterinary Sciences | ||
| Article 3, Volume 33, Issue 2, 2019, Pages 189-194 PDF (939.18 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/ijvs.2019.125523.1045 | ||
| Authors | ||
| E.R. Sarhat1; S. A. Wadi* 2; B.I. Sedeeq3; Th. R. Sarhat4; N.A. Jasim2 | ||
| 1Department of Basic Science, Dentistry College, University of Tikrit, Tikrit - Iraq | ||
| 2Department of Pharmacology, Collage of Veterinary Medicine, University of Tikrit, Tikrit - Iraq | ||
| 3Department of Basic Science, Dentistry College, University of Baghdad, Baghdad, Iraq | ||
| 4Department of Biology, College of Education, University of Baghdad, Baghdad - Iraq. | ||
| Abstract | ||
| This study was undertaken to determine the antidiabetic effects of oral administration of Punica granatum L. extract on serum and tissues of streptozotocin induced diabetic rabbits at 100 mg/kg. The present study was carried out at the Faculty of veterinary Medicine, Tikrit University, from February to August 2017 for 10 weeks. For this purpose, 30 rabbits were randomly separated into three groups, each containing 10 animals: Group 1, healthy control rabbits; Group 2, diabetic rabbits received streptozotocin (STZ, 65 mg/kg); Group 3, diabetic rabbits treated with PS extract (the 100 mg PS+1 ml DW) for 21 days. At the end of experiment, blood samples were taken for measuring serum biochemical parameters. For histopathological evaluation, sections of kidneys were fixed in 10% buffered formalin and 5micron thick sections with H&E stain were prepared using routine histopathological techniques. The treatment revealed that PSE extract significant decreased serum glucose throm-bospondin-1, nitric oxide, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase alkaline phosphatase, and C-reactive protein in diabetic treated rabbits as compared to diabetic rabbits. Histopathology of kidney showed lesions similar to human glomeruloscleroses, glomerular membrane thickening, arteriolar hyalinization and tubular necrosis. From the above one can conclude that PSE extract possess nephroprotective effect in experimentally induced diabetic rabbits. | ||
| Keywords | ||
| Histopathology; Punica granatum L; Extract; Rabbits | ||
|
Statistics Article View: 1,600 PDF Download: 1,245 |
||