Al-Rawi, M., Anis, , Essam, , F.Al-Jumaily, , Al-Saddi, H., Narjis, (2009). Extraction and purification of Urease from Proteus mirabilis. , 0(33), 138-145.
M. Al-Rawi; Anis; Essam; F.Al-Jumaily; H.M. Al-Saddi; Narjis. "Extraction and purification of Urease from Proteus mirabilis". , 0, 33, 2009, 138-145.
Al-Rawi, M., Anis, , Essam, , F.Al-Jumaily, , Al-Saddi, H., Narjis, (2009). 'Extraction and purification of Urease from Proteus mirabilis', , 0(33), pp. 138-145.
Al-Rawi, M., Anis, , Essam, , F.Al-Jumaily, , Al-Saddi, H., Narjis, Extraction and purification of Urease from Proteus mirabilis. , 2009; 0(33): 138-145.

Extraction and purification of Urease from Proteus mirabilis

Iraqi National Journal Of Chemistry
Article 1, Volume 0, Issue 33, 2014, Pages 138-145
Authors
M. Al-Rawi; ; ; ; H.M. Al-Saddi;
Abstract
Abstract
Urease was extracted from uropathogenic P.mirabilis using Lauria agar and then purified by ion- exchange chromatography using DEAE-cellulose and gel filtration chromatography on Sephacryl S-200 column. The purification fold was 13.86 and the yield was 45.4%.
The molecular weight of urease was estimated by gel filtration and SDS-polyacrylamide gel electrophoresis. The native molecular weight of the enzyme using gel filtration was about 174000 Daltons whereas SDS-polyacrylamide gel electrophoresis revealed three bands and the molecular weight for these bands were (53.70, 31.68, 20.89) kDa respectively.
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