Study of the inhibitory effect of some thiourea compounds on the activity of enzyme purified polyamine oxidase from the blood of patients with type 1 diabetes

Abbou, Nashwan Ibrahim; Al.Fleih, Khawla; Hamid, Eyad Saadi

doi:10.33899/edusj.2013.163091

	Study of the inhibitory effect of some thiourea compounds on the activity of enzyme purified polyamine oxidase from the blood of patients with type 1 diabetes
Journal of Education and Science
Article 31, Volume 26, Issue 5, 2013, Pages 74-87 PDF (699.15 K)
Document Type: Research Paper
DOI: 10.33899/edusj.2013.163091
Authors
Nashwan Ibrahim Abbou^* ¹; Khawla Al.Fleih^* ²; Eyad Saadi Hamid^* ³
¹Chemistry department, College of Education, University of Mosul, Mosul Iraq
²Chemistry department, College of Education for Girls, University of Mosul, Mosul Iraq
³Chemistry department, College of Science, University of Tikrit
Abstract
ABSTRACT The research included determination of polyamine oxidase (PAO) activity in normal and diabeti patients type I females. It was found that the activity of PAO in red blood cells (RBC) and plasma in patients were significantly higher than that of normal(p<0.05). The partial purification of PAO from RBC of diabetic and normal females were included in this study. This was achieved by using different biochemical techniques. Three proteinous peaks with PAO activities in RBC (I,II,III) from each of normal and diabetic with specific activities (U\mg protein) (0.194,0.183 and 0.098), (0.349, 0.237 and 0.176) respectively were isolated from ion exchange chromatography, with molecular weights (72588, 74512 and 69339), (141458, 100671 and 104018) Da respectively. This study didn't show the existence of Cu²⁺ ion as a cofactor for any PAO isoenzymes, but indicated the existence of FAD as a cofactor for all PAO isoenzymes. On the other hand the research included study the effect of some thiourea derivatives which contains semicarbazide compound , N[(Benzoylamino)]thioxymethyl-semicarbazide and N[(p-toluine sulfonylamino)] thioxymethyl-semicarbazide, and N[(p-toluine sulfonylamino)] thioxymethyl-hexylamine which contained hexylamine . Semicarbazide showed inhibitory effect on partially purified PAO activity. Thiourea derivatives from Semicarbazide showed competitive inhibition of PAO activity but with hexylamine was non- competitive.
Keywords
Inhibitory effect; thiourea compounds; polyamine oxidase; blood of patients with type 1 diabetes

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