- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Spectrophotometric determination of chloramphenicol (after reduction) by nitrogen and conjugation using fluorochlosinol reagent - application to eye drops | ||
| Journal of Education and Science | ||
| Article 36, Volume 26, Issue 5, 2013, Pages 138-155 PDF (713.75 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33899/edusj.2013.163096 | ||
| Authors | ||
| Waadallah Bashir* 1; Tamador Abbas Hamoudi* 2 | ||
| 1Department of Chemistry / College of Science / University of Mosul | ||
| 2College of Medicine Nineveh / University of Mosul | ||
| Abstract | ||
| ABSTRACT A spectrophotometric method for the assay of micro amounts of chloramphenicol was developed. This method is based on the reduction of the nitro-group of chloramphenicol to amino-group, followed by diazotization and coupling with phloroglucinol in alkaline medium to form a stable yellow azo dye which shows maximum absorption at 419 nm. Beer's law is obeyed over the concentration range (0.4 – 40) ppm. The molar absorptivity is (1.5437×104) l.mol-1.cm-1 and Sandell sensitivity index of (0.021) µg / cm2with a relative error of -1.8 to -0.52% and a relative standard deviation of ± 4.0 to ± 0.69%. The propose method has been applied to the determination of chloramphenicol in eye drop. | ||
| Keywords | ||
| Spectrophotometric determination; Chloramphenicol; fluorochlosinol reagent; eye drops | ||
|
Statistics Article View: 1,079 PDF Download: 299 |
||