Effect of diphenhydramine and phenobarbital in the concentration of glutathione and malondialdehyde and glucose in plasma and brain of chicks treated with pentylenetetrazol

Fadel, Maab; Mohammad, Fouad K.

doi:10.33899/ijvs.2019.163173

	Effect of diphenhydramine and phenobarbital in the concentration of glutathione and malondialdehyde and glucose in plasma and brain of chicks treated with pentylenetetrazol
Iraqi Journal of Veterinary Sciences
Article 53, Volume 33, Issue 2, 2019, Pages 97-104 PDF (677.37 K)
Document Type: Research Paper
DOI: 10.33899/ijvs.2019.163173
Authors
Maab Fadel^* ¹; Fouad K. Mohammad²
¹Department of Physiology, Biochemistry & Pharmacology, Collage of Veterinary Medicine, University of Mosul, Mosul, Iraq
²Professor of Veterinary Sciences, University of Mosul
Abstract
The aim of this study was to develop convulsion by giving pentylenetetrazol and to observe the interaction of this drug with diphenhydramine and phenobarbital in chickens with an evaluation of the interaction of these drugs and their effect on the biochemical parameters. The chicks were divided into five groups, each group containing six birds, control group injected with normal saline at 2ml/kg, second group injected with PTZ at56.5 mg/kg I.P., third group injected with diphenhydramine at dose 2.5 mg/kg I.M., before 20 minutes from injected of PTZ at dose 56.5 mg/kg I.P., forth group injected with phenobarbital at dose 10 mg /kg I.M., before 20 minutes from injected of PTZ at dose 56.5 mg/kg I.P., fifth group injected with diphenhydramineat dose 2.5 mg/kgI.M. and phenobarbital at dose 10 mg /kg I.M. before 20 minutes from injection of PTZ at dose 56.5 mg/kgI.P. The results showed that group which was treated with PTZ alone showed a significant decrease in glutathione concentration in plasma and brain compare with the control group. All groups except the group treated with PTZ diphenhydramine and phenobarbital showed a significantly increased in malondialdehyde concentration in plasma and brain compare with control group, as well as, all groups except the group treated with PTZ and phenobarbital appeared significantly increased in glucose concentration in plasma compared with the control group. In conclusion, the study appeared oxidative stress result in the lipid peroxidation which main reasons for decreasing of glutathione concentration and increasing of malondialdehyde concentration in chicks.
Keywords
Pentylenetetrazol; Phenobarbital; antiseizure drug

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