Flow Injection Spectrophotometric and Reverse Phase-High Performance Liquid Chromatography Determination of Ranitidine.Hydrochlrid inPharmacetical Preparations.

A. Mustafa, Mohammed; Q. Al-Abachi, Mouayed; J.M. Hussan, Mohammed.

	Flow Injection Spectrophotometric and Reverse Phase-High Performance Liquid Chromatography Determination of Ranitidine.Hydrochlrid inPharmacetical Preparations.
Iraqi National Journal Of Chemistry
Article 1, Volume 0, Issue 22, 2014, Pages 189-200
Authors
Mohammed A. Mustafa; Mouayed Q. Al-Abachi; Mohammed. J.M. Hussan
Abstract
Abstract This study describes development of a new flow injection spectrophotometric method and a reverse phase-high performance liquid chromatography RP-HPLC method for the determination of Ranitidine.HCl (RH) in aqueous solution and in pharmaceutical preparations. The FIA method was based on the reaction of RH with ferric nitrate and the produced Fe(II) was reacted with potassium hexacyanoferrate (III) forming a Prussian blue colored dye that has a maximum absorption at 800 nm. Linearity was in the range of 2-80 g.ml-1, correlation coefficient of 0.9996, detection limit of 0.613 g.ml-1 and relative standard deviation percent of 2.17%. The method was applied successfully to the determination of RH in pharmaceutical preparations with a recovery of 100.23 – 101.04%. RP-HPLC method with a Supelco BDS-C18 –DS (25 cm x 46 mm i.d) analytical column (m particle size) and isocratic elution with a mobile phase containing 30% methanol in 0.05 M KH2PO4 buffer (pH 6.0), at a flow rate of 1 ml.min-1, 20 L sample loop, and a UV detector at max 330 nm. The calibration graph was linear in the range of 0.1 – 1.5 g.ml-1 with the correlation coefficient of 0.9994. The method was applied successfully for the determination of pharmaceutical preparations containing Ranitidine.HCl with a recovery of 100.63–101.31%.

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