- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
The role of plasmid stability and the associated resistant gene in the antimicrobial resistance of Fusobacterium spp. Isolates from chronic periodontitis | ||
| Journal of baghdad college of dentistry | ||
| Article 1, Volume 21, Issue 2, 2009, Pages 87-90 | ||
| Author | ||
| Ali A. Abdulkareem | ||
| Abstract | ||
| ABSTRACTBackground: The microbial composition of dental plaque is highly diverse. Fusobacterium spp. is gram negative, nonmotile,anaerobic rods that are consider as part of the normal flora of oral cavity playing a major role in initiation &progression of periodontal diseases. The purpose of the present study is to evaluate the plasmid stability & theassociation of the resistance gene with the plasmid in oral Fusobacterium spp. Isolated from subjects with chronicperiodontitisMaterials and Method: A total of 11 Fusobacterium isolates were taken from 32 subjects with chronic periodontitiswith average pocket depth range between 6- 8mm. Antibiotic susceptibility to 12 antimicrobials, DNA extraction,plasmid profile analysis, curing, & conjugation & transformation were carried out on these isolates.Results: The isolates showed variable degree of resistance to different antimicrobials but all were susceptible toDoxycycline. Plasmid analysis of isolates, three major plasmid patterns were observed 3.1, 4.9 and 6.2-kb. In curingexperiments, resistance to Erythromycin, Penicillin, and Clarithromycin were lost together and their loss wasaccompanied by the loss of a plasmid of 3.1-kb. Similarly, resistance was accompanied by the loss of a 6.2 -kbplasmid. In transfer experiments, no resistance determinants were transferred by conjugation, indicating that they didnot harbor conjugative plasmid.Conclusion: The antimicrobial resistance among isolates is either plasmid- related for certain antibiotics orchromosomally- mediated for the others.Key words: Chronic periodontitis, Fusobacterium nucleatum, microbiology of periodontitis. J Bagh Coll Dentistry 2009;21(2):87-90) | ||
|
Statistics Article View: 173 PDF Download: 27 |
||