Isihak, F. (2020). Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province. , 34(1), 77-81. doi: 10.33899/ijvs.2019.125469.1007
Fanar Isihak. "Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province". , 34, 1, 2020, 77-81. doi: 10.33899/ijvs.2019.125469.1007
Isihak, F. (2020). 'Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province', , 34(1), pp. 77-81. doi: 10.33899/ijvs.2019.125469.1007
Isihak, F. Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province. , 2020; 34(1): 77-81. doi: 10.33899/ijvs.2019.125469.1007

Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province

Iraqi Journal of Veterinary Sciences
Article 12, Volume 34, Issue 1, 2020, Pages 77-81    PDF (1.16 M)
Document Type: Research Paper
DOI: 10.33899/ijvs.2019.125469.1007
Author
Fanar Isihak*
Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq
Abstract
Avian reoviruses can infect birds without any clinical signs of infection, the infection may associate with different manifestations including viral arthritis/tenosynovitis and malabsorption syndrome. The objective of this study was to use advance methods representing by molecular methods (RT-PCR, RT-qPCR) in the diagnosis of ARV infection in broiler breeders' flocks. A 4 flocks of broiler breeders (ROSS breed) 39 weeks age with approximately10% morbidity rate due to Avian Reovirus (ARV). The clinical examination of 16 infected birds revealed unilateral lameness and swelling of hock joint. Blood samples were collected from wing vein of infected birds. Sera were tested for antibodies titer against ARV and Mycoplasma synoviae (MS). 5 of 16 positive samples were selected randomly for amplification by RT-PCR and RT-qPCR. The results showed in postmortem examination of infected birds, unilateral arthritis with visible joint lesions. Antibodies titer measured by ELISA in the sera of birds after 4 and 20 weeks of infection with ARV was positive and high. In RT- PCR1 of 5 samples gave positive reaction for amplification while in RT-qPCR all five samples gave positive results for amplification in comparison with +ve and -ve control.
Keywords
Reovirus; Broiler Breeders; RT-PCR; RT-qPCR
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