Spectrophotometric Method for Estimation of Promethazine- HCl in Pharmaceutical Formulation Based on Oxidative Coupling with the Reagent m-Amino Benzoic Acid in Presence of N-Bromosuccinimide.

Abdulaziz, Mohammad Salim; Shareef, Aseen N.; Bakir, Mohsin Hamza

doi:10.32894/kujss.2019.14.4.14

	Spectrophotometric Method for Estimation of Promethazine- HCl in Pharmaceutical Formulation Based on Oxidative Coupling with the Reagent m-Amino Benzoic Acid in Presence of N-Bromosuccinimide.
Kirkuk Journal of Science
Article 1, Volume 14, Issue 4, 2019, Pages 236-256 PDF (0 K)
Document Type: Research Paper
DOI: 10.32894/kujss.2019.14.4.14
Authors
Mohammad Salim Abdulaziz; Aseen N. Shareef^* ; Mohsin Hamza Bakir
Department of Chemistry, College of Education for Women, Tikrit University.
Abstract
An easy, rapid and sensitive novel spectrophotometric method for estimation of trace amounts of Promethazine-HCl in aqueous solution is described. The method is based on using the oxidative coupling reaction of Promethazine-HCl with m-amino benzoic acid reagent at pH1.2 in the presence of N-bromosuccinimide to produce an intense green color, water soluble, product which exhibit maximum absorbance at 592 nm. Beer's law is obeyed over concentration range of (2-26 µg/ml), with a Ԑ 9.754×〖10〗^3 l/mol.cm, Coefficient determination (R2=0.9991). Sandell̕s index of 0.03289 µg/cm^2, the average recovery is 100.396 %, RSD does not exceed 0.3096 %, LOD of 0.1949 µg/m1 and LOQ of 0.5905 µg/m1. The proposed method was applied successfully for determination of PMZH in its pharmaceutical formulations..
Keywords
Mohammad Salim Abdulaziz; Aseen N; Shareef; Mohsin Hamza Bakir

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