Bacterial Contamination and its Response to Antibiotics and Disinfectants Used in the Children's Hospital in Kirkuk

Mohamed, Ahmed A.; Mahde, Najdat B.

doi:10.32894/kujss.2019.14.2.11

	Bacterial Contamination and its Response to Antibiotics and Disinfectants Used in the Children's Hospital in Kirkuk
Kirkuk Journal of Science
Article 1, Volume 14, Issue 2, 2019, Pages 175-192 PDF (0 K)
Document Type: Research Paper
DOI: 10.32894/kujss.2019.14.2.11
Authors
Ahmed A. Mohamed^* ; Najdat B. Mahde
Department of Biology, College of Science, University of Kirkuk ,Kirkuk ,Iraq.
Abstract
The study included, the collection of (250) swabs which involve (180) samples from general children's Hospital environment and various isolation sources, In addition to (70) smear from the hands and noses of medical staffs and workers, Using sterile cotton swabs and then planted on the Cultuer media (MacConkey's agar and Blood agar). The results showed that (126) smears and in a ratio of (50.4%) was positive for the bacterial isolations which contribute the followings:- Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli , Enterobacter cloacae, Proteus mirabilis, Klebsiella pneumonia, Pseudomonas aeruginosa, with variant isolation ratios. It high rate of contamination (73.33%), was from Hospitals environment compared to (71%) of contamination from the hands of medical staffs. Resistany agaings antibiotic was praceddon (12) antibiotics and the results showed that most of the isolates are resistant For anti-beta-lactam groups the rate was (81.74%), the followings antibiotics vancomycin, imipenem, amikacin reveal high efficucy against the isolated Staph.aureus ,Staph.epidermidis ,Esch.col, Prot.mirabilis , pathogens Ps.aeruginosa with reduction resistany reach to (23.80), (24.60), (36.50) respectively. The Minmam inhibitory concentration was detected using virkon, Opizil, Strillium, and bleach, ethyl alcohol, Optisal ® N was found to passing a better inhibition effect on the elected isolates, followed by an Virkon, compared with the disinfectant of sodium hyper chlorate (minor), The following value MIC walues were obtained (0.25-0.0625, 0.5-0.078, 1.25-0.156%) moreover, the antiseptics used for cleaning the hands showed that, a streillium antiseptics is wosh high bacterial inhibition impact than the ethyl alcohol, as the lower inhibitory values of the streillium antiseptics ranged from (80-50%) while the ethyl alcohol disinfectant was the least efficient as it reached its inhibitory values minimum (80-60). 80-60%)).
Keywords
Hospital Environment; Antiseptics; bacterial contamination; Escherichia coli

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